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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
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- Product number
- PA5-22165 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- STK40 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (92%), Chicken (92%), Chimpanzee (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.97 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Myc-DDK-tagged serine/threonine kinase 40 in Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg). Samples were separated by 10% SDS-PAGE and the membrane was probed with serine/threonine kinase 40 Polyclonal antibody (Product # PA5-22165) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using STK40 Polyclonal Antibody (Product # PA5-22165). Sample (30 µg of whole cell lysate). Lane A: Hela. Lane B: Hep G2. 10% SDS PAGE. STK40 Polyclonal Antibody (Product # PA5-22165) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using STK40 Polyclonal Antibody (Product # PA5-22165). Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with serine/threonine kinase 40 antibody [N3C3] STK40 Polyclonal Antibody (Product # PA5-22165) diluted at 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of serine/threonine kinase 40 in paraformaldehyde-fixed HeLa cells using a serine/threonine kinase 40 polyclonal antibody (Product # PA5-22165) at a 1:200 dilution.
