Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- PA5-46949 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NPTXR Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) NPTX2 and rhNPTX1 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of NPTXR in 0.2 mg/mL lysates of U‚87 MG human glioblastoma/astrocytoma cell line and human hippocampus tissue. Samples were incubated in NPTXR polyclonal antibody (Product # PA5-46949) using a dilution of 20 µg/mL followed by HRP-conjugated Anti-Sheep IgG at a dilution of 0.0763888888888889. A specific band was detected for Neuronal Pentraxin R/NPTXR at approximately 66 kDa (as indicated). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.