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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- MAB5846 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal FGF-4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse FGF-4 in direct ELISAs. In direct ELISAs, 10% - 50% cross-reactivity with recombinant mouse (rm) FGF-10, rmFGF-7, recombinant human (rh) FGF-16, rhFGF-20 and less than 5% cross-reactivity with rmFGF acidic, rmFGF basic, rmFGF-6, -8b, -8c, -15, -17, -21, -23, rhFGF acidic, rhFGF basic, rhFGF-3, -4, -5, -9, -11, -12, -13, -18, -19, or -22 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- FGF-4 in Mouse Embryo. FGF-4 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Rat Anti-Mouse FGF-4 Monoclonal Antibody (Catalog # MAB5846) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
