500-P158-100UG

antibody from Invitrogen Antibodies

Targeting: FGF4 HBGF-4, HST, HST-1, HSTF1, K-FGF, KFGF

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

500-P158-100UG

Provider

Invitrogen Antibodies

Product name

FGF4 Polyclonal Antibody, PeproTech®

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

AA Sequence of recombinant protein: GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL.

Concentration

0.1-1.0 mg/mL

References

Submitted references

Fibroblast growth factor 4 and its novel splice isoform have opposing effects on the maintenance of human embryonic stem cell self-renewal.

Mayshar Y, Rom E, Chumakov I, Kronman A, Yayon A, Benvenisty N
Stem cells (Dayton, Ohio) 2008 Mar;26(3):767-74

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect Human FGF-4 by Western Blot analysis FGF4 Polyclonal Antibody (Product # 500-P158-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human FGF-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA: To detect Human FGF-4 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of FGF4 Polyclonal Antibody (Product # 500-P158-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech FGF4 Polyclonal Antibody, Biotin (Product # 500-P158BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human FGF-4.