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Learn500-P158-1MG
antibody from Invitrogen Antibodies
Targeting: FGF4
HBGF-4, HST, HST-1, HSTF1, K-FGF, KFGF
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- ELISA [1]
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- Product number
- 500-P158-1MG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FGF4 Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human FGF-4. Anti-Human FGF-4-specific antibody was purified by affinity chromatography employing an immobilized Human FGF-4 matrix. Sandwich ELISA: To detect Human FGF-4 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human FGF-4 (500-P158Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human FGF-4. Western Blot: To detect Human FGF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human FGF-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 1 mg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references Fibroblast growth factor 4 and its novel splice isoform have opposing effects on the maintenance of human embryonic stem cell self-renewal.
Mayshar Y, Rom E, Chumakov I, Kronman A, Yayon A, Benvenisty N
Stem cells (Dayton, Ohio) 2008 Mar;26(3):767-74
Stem cells (Dayton, Ohio) 2008 Mar;26(3):767-74
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Sandwich ELISA: To detect Human FGF-4 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of FGF4 Polyclonal Antibody (Product # 500-P158-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech FGF4 Polyclonal Antibody, Biotin (Product # 500-P158BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human FGF-4.
