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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-42500 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FADS1 Recombinant Rabbit Monoclonal Antibody (JE55-63)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: Rat liver tissue lysate, mouse liver tissue lysate, mouse brain tissue lysate, rat lung tissue lysate, Hela cell lysate, A549 cell lysate, Hela, PANC-1, human liver carcinoma tissue, human thyroid tissue, human colon carcinoma tissue, human breast carcinoma tissue, human stomach carcinoma tissue, human small intestine tissue, A549. Subcellular Location: Mitochondrion, Endoplasmic reticulum membrane.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JE55-63
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FADS1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. FADS1 Recombinant Monoclonal Antibody (Product # MA5-42500) at 1:1,000 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: rat liver tissue lysate. Lane 2: mouse liver tissue lysate. Lane 3: mouse brain tissue lysate. Lane 4: rat lung tissue lysate. Lane 5: Hela cell lysate. Lane 6: A549 cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of of FADS1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with FADS1 Recombinant Monoclonal Antibody (Product # MA5-42500) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of of FADS1 in PANC-1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with FADS1 Recombinant Monoclonal Antibody (Product # MA5-42500) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of FADS1 in A549 cells using FADS1 Recombinant Monoclonal Antibody (Product # MA5-42500) at 1:50 (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1:1,000 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
