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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-30080 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FADS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HepG2, HCT116.
- Concentration
- 0.91 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FADS1 Polyclonal Antibody (Product # PA5-30080). Sample (30 µg of whole cell lysate). Lane A: A549 . 7.5% SDS PAGE. FADS1 Polyclonal Antibody (Product # PA5-30080) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FADS1 Polyclonal Antibody detects FADS1 protein at mitochondria by immunofluorescent analysis. Sample: Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: FADS1 protein stained by FADS1 Polyclonal Antibody (Product # PA5-30080) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using FADS1 (Product # PA5-30080) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
