PA5-26288

antibody from Invitrogen Antibodies

Targeting: SNRPD3 Sm-D3, SMD3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-26288

Provider

Invitrogen Antibodies

Product name

SNRPD3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with mouse and Xenopus based on sequence homology.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

A missense mutation in SNRPE linked to non-syndromal microcephaly interferes with U snRNP assembly and pre-mRNA splicing.

Chen T, Zhang B, Ziegenhals T, Prusty AB, Fröhler S, Grimm C, Hu Y, Schaefke B, Fang L, Zhang M, Kraemer N, Kaindl AM, Fischer U, Chen W
PLoS genetics 2019 Oct;15(10):e1008460

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Impaired spliceosomal UsnRNP assembly leads to Sm mRNA down-regulation and Sm protein degradation.

Prusty AB, Meduri R, Prusty BK, Vanselow J, Schlosser A, Fischer U
The Journal of cell biology 2017 Aug 7;216(8):2391-2407

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SNRPD3 in MCF-7, Jurkat cell line lysates. Samples were incubated with SNRPD3 polyclonal antibody (Product # PA5-26288). Lysates: 35 µg/lane. SNRPD3 (arrow).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SNRPD3 in formalin fixed and paraffin embedded human lung carcinoma. Samples were incubated with SNRPD3 polyclonal antibody (Product # PA5-26288) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SNRPD3 in formalin-fixed and paraffin-embedded human kidney tissue. Samples were incubated with SNRPD3 polyclonal antibody (Product # PA5-26288). This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SNRPD3 in formalin fixed and paraffin embedded human lung carcinoma. Samples were incubated with SNRPD3 polyclonal antibody (Product # PA5-26288) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of MCF-7 cells using a SNRPD3 polyclonal antibody (Product # PA5-26288) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of SNRPD3 in MCF-7 cells (bottom histogram). Samples were incubated with SNRPD3 polyclonal antibody (Product # PA5-26288) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (top histogram).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 3 NSM mutation causes mis-localization of the SNRPE/SmE protein. (A-B), Indirect immunofluorescence and confocal microscopy of HeLa cells transfected with either FLAG-tagged wild type or mutant SmE (WT/Mut) or left untransfected (negative control). Empty white arrowheads indicate localization pattern observed and filled white arrowheads indicate zoomed in region shown in the overly inset. (A), Top panel shows clear co-localization of wild type SmE (green) and coilin (magenta) in CBs and middle panel shows most of the mutant SmE (green) distributed in the cytoplasm with a minor fraction in the nucleus and co-localizing with coilin (magenta) in CBs. (B), While wild type SmE (green, top panel) co-localizes with SmD3 (magenta) in CBs and splicing speckles, the mutant SmE (green, middle panel) is predominantly cytoplasmic with marginal co-localization with SmD3 in CBs or in nuclear speckles.