10365-1-AP

antibody from Proteintech Group

Targeting: DKK3 REIC, RIG

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

10365-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#10365-1-AP, RRID:AB_2261615

Product name

DKK3 antibody

Antibody type

Polyclonal

Description

KD/KO validated DKK3 antibody (Cat. #10365-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: FC, IF, IHC, WB, ELISA.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

A single cell atlas of frozen shoulder capsule identifies features associated with inflammatory fibrosis resolution.

Ng MTH, Borst R, Gacaferi H, Davidson S, Ackerman JE, Johnson PA, Machado CC, Reekie I, Attar M, Windell D, Kurowska-Stolarska M, MacDonald L, Alivernini S, Garvilles M, Jansen K, Bhalla A, Lee A, Charlesworth J, Chowdhury R, Klenerman P, Powell K, Hackstein CP, ICECAP Consortium, Furniss D, Rees J, Gilroy D, Coles M, Carr AJ, Sansom SN, Buckley CD, Dakin SG
Nature communications 2024 Feb 19;15(1):1394

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DKK3/CKAP4 axis is associated with advanced stage and poorer prognosis in oral cancer.

Katase N, Kudo K, Ogawa K, Sakamoto Y, Nishimatsu SI, Yamauchi A, Fujita S
Oral diseases 2023 Nov;29(8):3193-3204

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Immuno-histochemical correlation of fibrosis-related markers with the desmoplastic reaction of the mesentery in small intestine neuroendocrine neoplasms.

Bösch F, Altendorf-Hofmann A, Koliogiannis V, Ilhan H, Jacob S, Pretzsch E, Nölting S, Werner J, Klauschen F, Auernhammer CJ, Angele MK, Knösel T
Journal of cancer research and clinical oncology 2023 May;149(5):1895-1903

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Deep Sc-RNA sequencing decoding the molecular dynamic architecture of the human retina.

Huang L, Li R, Ye L, Zhang S, Tian H, Du M, Qu C, Li S, Li J, Yang M, Wu B, Chen R, Huang G, Zhong L, Yang H, Yu M, Shi Y, Wang C, Zhang H, Chen W, Yang Z
Science China. Life sciences 2023 Mar;66(3):496-515

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Single-cell multimodal analysis identifies common regulatory programs in synovial fibroblasts of rheumatoid arthritis patients and modeled TNF-driven arthritis.

Armaka M, Konstantopoulos D, Tzaferis C, Lavigne MD, Sakkou M, Liakos A, Sfikakis PP, Dimopoulos MA, Fousteri M, Kollias G
Genome medicine 2022 Jul 26;14(1):78

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Retinoic acid effects on in vitro palatal fusion and WNT signaling.

Roa Fuentes LA, Bloemen M, Carels CE, Wagener FA, Von den Hoff JW
European journal of oral sciences 2022 Dec;130(6):e12899

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Exercise ameliorates insulin resistance and improves ASK1-mediated insulin signalling in obese rats.

Zhang Y, Ye T, Zhou P, Li R, Liu Z, Xie J, Hua T, Sun Q
Journal of cellular and molecular medicine 2021 Dec;25(23):10930-10938

---

MicroRNA miR-425 promotes tumor progression by inhibiting Dickkopf-related protein-3 in gastric cancer.

Pei Y, Tang Z, Cai M, Yao Q, Xie B
Bioengineered 2021 Dec;12(1):2045-2054

---

Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.

Wang L, Yu P, Zhou B, Song J, Li Z, Zhang M, Guo G, Wang Y, Chen X, Han L, Hu S
Nature cell biology 2020 Jan;22(1):108-119

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miR-1303 promotes the proliferation, migration and invasion of prostate cancer cells through regulating the Wnt/β-catenin pathway by targeting DKK3.

Liu B, Zhou W, Jiang H, Xiang Z, Wang L
Experimental and therapeutic medicine 2019 Dec;18(6):4747-4757

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Bone secreted factors induce cellular quiescence in prostate cancer cells.

Yu-Lee LY, Lee YC, Pan J, Lin SC, Pan T, Yu G, Hawke DH, Pan BF, Lin SH
Scientific reports 2019 Dec 9;9(1):18635

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Suppression of stromal-derived Dickkopf-3 (DKK3) inhibits tumor progression and prolongs survival in pancreatic ductal adenocarcinoma.

Zhou L, Husted H, Moore T, Lu M, Deng D, Liu Y, Ramachandran V, Arumugam T, Niehrs C, Wang H, Chiao P, Ling J, Curran MA, Maitra A, Hung MC, Lee JE, Logsdon CD, Hwang RF
Science translational medicine 2018 Oct 24;10(464)

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MicroRNA-183 promotes the proliferation and metastasis of renal cell carcinoma through targeting Dickkopf-related protein 3.

Zhang XL, Xu G, Zhou Y, Yan JJ
Oncology letters 2018 Apr;15(4):6003-6008

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Dickkopf 3 (Dkk3) Improves Amyloid-β Pathology, Cognitive Dysfunction, and Cerebral Glucose Metabolism in a Transgenic Mouse Model of Alzheimer's Disease.

Zhang L, Sun C, Jin Y, Gao K, Shi X, Qiu W, Ma C, Zhang L
Journal of Alzheimer's disease : JAD 2017;60(2):733-746

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Canine REIC/Dkk-3 interacts with SGTA and restores androgen receptor signalling in androgen-independent prostate cancer cell lines.

Kato Y, Ochiai K, Kawakami S, Nakao N, Azakami D, Bonkobara M, Michishita M, Morimatsu M, Watanabe M, Omi T
BMC veterinary research 2017 Jun 9;13(1):170

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Properties of the feline tumour suppressor reduced expression in immortalized cells (REIC/Dkk-3).

Ochiai K, Oda H, Shono S, Kato Y, Sugihara S, Nakazawa S, Azakami D, Michishita M, Onozawa E, Bonkobara M, Sako T, Shun-Ai L, Ueki H, Watanabe M, Omi T
Veterinary and comparative oncology 2017 Dec;15(4):1181-1186

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Phosphatase WIP1 regulates adult neurogenesis and WNT signaling during aging.

Zhu Y, Demidov ON, Goh AM, Virshup DM, Lane DP, Bulavin DV
The Journal of clinical investigation 2014 Jul;124(7):3263-73

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Molecular cloning and tumour suppressor function analysis of canine REIC/Dkk-3 in mammary gland tumours.

Ochiai K, Watanabe M, Azakami D, Michishita M, Yoshikawa Y, Udagawa C, Metheenukul P, Chahomchuen T, Aoki H, Kumon H, Morimatsu M, Omi T
Veterinary journal (London, England : 1997) 2013 Sep;197(3):769-75

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SOX2 promotes tumor metastasis by stimulating epithelial-to-mesenchymal transition via regulation of WNT/β-catenin signal network.

Li X, Xu Y, Chen Y, Chen S, Jia X, Sun T, Liu Y, Li X, Xiang R, Li N
Cancer letters 2013 Aug 19;336(2):379-89

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microRNA-183 is an oncogene targeting Dkk-3 and SMAD4 in prostate cancer.

Ueno K, Hirata H, Shahryari V, Deng G, Tanaka Y, Tabatabai ZL, Hinoda Y, Dahiya R
British journal of cancer 2013 Apr 30;108(8):1659-67

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Wnt antagonist DICKKOPF-3 (Dkk-3) induces apoptosis in human renal cell carcinoma.

Ueno K, Hirata H, Majid S, Chen Y, Zaman MS, Tabatabai ZL, Hinoda Y, Dahiya R
Molecular carcinogenesis 2011 Jun;50(6):449-57

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 10365-1-AP( DKK3 Antibody) at dilution of 1:1000

Sample type

cell line

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

mouse brain tissue were subjected to SDS PAGE followed by western blot with 10365-1-AP(DKK3 antibody) at dilution of 1:500

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver cancer using 10365-1-AP(DKK3 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The DKK3 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human DKK3. This antibody recognizes human, mouse antigen. The DKK3 antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

10365-1-AP.pdf

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