Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- BAF1118 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Dkk-3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Dkk-3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse (rm) Dkk-1, rmDkk-3, rmDkk-4, recombinant human (rh) Dkk-1, and rhDkk-4 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Dkk-3 is elevated in CSF and plasma of Alzheimer's disease patients.
Zenzmaier C, Marksteiner J, Kiefer A, Berger P, Humpel C
Journal of neurochemistry 2009 Jul;110(2):653-61
Journal of neurochemistry 2009 Jul;110(2):653-61
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Dkk-3 in Human Lung. Dkk-3 was detected in immersion fixed paraffin-embedded sections of human lung array using Goat Anti-Human Dkk-3 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1118) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Dkk-3 in Human Lung. Dkk-3 was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human Dkk-3 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1118) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.