PA5-30525

antibody from Invitrogen Antibodies

Targeting: DKK3 REIC, RIG

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30525

Provider

Invitrogen Antibodies

Product name

DKK3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, Jurkat, Raji. Predicted reactivity: Mouse (84%), Rat (81%), Dog (86%), Pig (86%), Rhesus Monkey (97%), Bovine (88%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using DKK3 Polyclonal Antibody (Product # PA5-30525). Sample (30 µg of whole cell lysate). Lane A: 293T. 10% SDS PAGE. DKK3 Polyclonal Antibody (Product # PA5-30525) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of DKK3 was performed in DIV10 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: DKK3 Polyclonal Antibody (Product # PA5-30525) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Frozen) analysis of DKK3 was performed in frozen-sectioned mouse cerebral cortex tissue using DKK3 Polyclonal Antibody (Product # PA5-30525) at a dilution of 1:250 (Green). Blue: Fluoroshield with DAPI.