Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- HPA011868 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA011868, RRID:AB_1847716
- Product name
- Anti-DKK3
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human DKK3, Gene description: dickkopf WNT signaling pathway inhibitor 3, Alternative Gene Names: REIC, RIG, Validated applications: WB, IHC, ICC, Uniprot ID: Q9UBP4, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Rituximab versus tocilizumab in rheumatoid arthritis: synovial biopsy-based biomarker analysis of the phase 4 R4RA randomized trial
Rivellese F, Surace A, Goldmann K, Sciacca E, Çubuk C, Giorli G, John C, Nerviani A, Fossati-Jimack L, Thorborn G, Ahmed M, Prediletto E, Church S, Hudson B, Warren S, McKeigue P, Humby F, Bombardieri M, Barnes M, Lewis M, Pitzalis C, Rivellese F, Giorli G, Nerviani A, Fossati-Jimack L, Thorborn G, Humby F, Bombardieri M, Lewis M, Durez P, Buch M, Rizvi H, Mahto A, Montecucco C, Lauwerys B, Ng N, Ho P, Romão V, da Fonseca J, Verschueren P, Kelly S, Sainaghi P, Gendi N, Dasgupta B, Cauli A, Reynolds P, Cañete J, Ramirez J, Celis R, Moots R, Taylor P, Edwards C, Isaacs J, Sasieni P, Choy E, Thompson C, Bugatti S, Bellan M, Congia M, Holroyd C, Pratt A, White L, Warren L, Peel J, Hands R, Hadfield G, Pitzalis C
Nature Medicine 2022;28(6):1256-1268
Nature Medicine 2022;28(6):1256-1268
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and DKK3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415712).
- Sample type
- Human
- Protocol
- Protocol