NB300-578

antibody from Novus Biologicals

Targeting: ATP2B1 PMCA1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB300-578

Provider

Novus Biologicals

Proper citation

Novus Cat#NB300-578, RRID:AB_2091527

Product name

Mouse Monoclonal PMCA1 Antibody

Antibody type

Monoclonal

Description

Unpurified. This detects all four known isoforms of the PMCA ATPase.

Reactivity

Human, Mouse, Rat, Canine, Chicken/Avian, Feline, Hamster, Porcine, Rabbit, Sheep, Simian

Host

Mouse

Antigen sequence

This antibody recognizes an epitope
between amino acids 724-783 of the
human erythrocyte calcium pump.


Isotype

IgG

Vial size

100uL

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

Evidence for a regulated Ca(2+) entry in proximal tubular cells and its implication in calcium stone formation.

Ibeh CL, Yiu AJ, Kanaras YL, Paal E, Birnbaumer L, Jose PA, Bandyopadhyay BC
Journal of cell science 2019 Apr 30;132(9)

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Sodium accumulation promotes diastolic dysfunction in end-stage heart failure following Serca2 knockout.

Louch WE, Hougen K, Mørk HK, Swift F, Aronsen JM, Sjaastad I, Reims HM, Roald B, Andersson KB, Christensen G, Sejersted OM
The Journal of physiology 2010 Feb 1;588(Pt 3):465-78

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Maitotoxin converts the plasmalemmal Ca(2+) pump into a Ca(2+)-permeable nonselective cation channel.

Sinkins WG, Estacion M, Prasad V, Goel M, Shull GE, Kunze DL, Schilling WP
American journal of physiology. Cell physiology 2009 Dec;297(6):C1533-43

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Splice-site A choice targets plasma-membrane Ca2+-ATPase isoform 2 to hair bundles.

Hill JK, Williams DE, LeMasurier M, Dumont RA, Strehler EE, Gillespie PG
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Jun 7;26(23):6172-80

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Expression of calcium transporters in the retina of the tiger salamander (Ambystoma tigrinum).

Krizaj D, Liu X, Copenhagen DR
The Journal of comparative neurology 2004 Aug 2;475(4):463-80

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Plasma membrane Ca2+ pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies.

Filoteo AG, Elwess NL, Enyedi A, Caride A, Aung HH, Penniston JT
The Journal of biological chemistry 1997 Sep 19;272(38):23741-7

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Plasma membrane Ca2+ pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies.

Filoteo AG, Elwess NL, Enyedi A, Caride A, Aung HH, Penniston JT
The Journal of biological chemistry 1997 Sep 19;272(38):23741-7

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Detection of isoform 4 of the plasma membrane calcium pump in human tissues by using isoform-specific monoclonal antibodies.

Caride AJ, Filoteo AG, Enyedi A, Verma AK, Penniston JT
The Biochemical journal 1996 May 15;316 ( Pt 1):353-9

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Detection of isoform 4 of the plasma membrane calcium pump in human tissues by using isoform-specific monoclonal antibodies.

Caride AJ, Filoteo AG, Enyedi A, Verma AK, Penniston JT
The Biochemical journal 1996 May 15;316 ( Pt 1):353-9

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Plasma membrane calcium pump and 28-kDa calcium binding protein in cells of rat kidney distal tubules.

Borke JL, Caride A, Verma AK, Penniston JT, Kumar R
The American journal of physiology 1989 Nov;257(5 Pt 2):F842-9

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PMCA1 Antibody (5F10) [NB300-578] - Analysis of 25 ug of U251 (lane 1), human brain (lane 2) and C2C12 (lane 3).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human heart tissues.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase in Jurkat cells (green) compared to an isotype control (blue).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase in Hela cells (green) compared to an isotype control (blue).