Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- AP31808PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP31808PU-N, RRID:AB_11147349
- Product name
- anti MAP-2
- Antibody type
- Polyclonal
- Antigen
- Two synthetic peptides KLH conjugatedcorresponding to different regions of the MAP-2 gene product, but are shared between the Human (NP_002365) and Mouse (P20357) sequences.Production: After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks.These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/ml. Finally, equal volumes of both of these affinity purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
- Reactivity
- Human, Mouse
- Host
- Chicken/Avian
- Vial size
- 0.2 mg
- Concentration
- 0.2 mg/ml (based on Bradford assay readings using BSA as a standard).
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Dissociated cell cultures of an e13 Mouse brain showing MAP-2 (green staining) of neurons. DAPI (blue staining) allows visualization of nuclei.