A01201-1

antibody from Boster Biological Technology

Targeting: MAP2 MAP2A, MAP2B, MAP2C

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

A01201-1

Provider

Boster Biological Technology

Product name

Anti-MAP2 Antibody

Antibody type

Polyclonal

Description

Rabbit Polyclonal antibody for MAP2 detection. Tested positive for WB, IF in Human, Mouse, Rat.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100ul

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

References

Submitted references

Neural Stem Cell-Derived Exosomes Improve Neurological Function in Rats with Cerebral Ischemia-Reperfusion Injury by Regulating Microglia-Mediated Inflammatory Response.

Zhao X, Zhu J, Chen S, Liu R, Long T
Journal of inflammation research 2023;16:3079-3092

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Bu Shen Huo Xue decoction promotes functional recovery in spinal cord injury mice by improving the microenvironment to promote axonal regeneration.

Hou Y, Luo D, Hou Y, Luan J, Zhan J, Chen Z, E S, Xu L, Lin D
Chinese medicine 2022 Jul 12;17(1):85

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Tauroursodeoxycholic acid alleviates secondary injury in spinal cord injury mice by reducing oxidative stress, apoptosis, and inflammatory response.

Hou Y, Luan J, Huang T, Deng T, Li X, Xiao Z, Zhan J, Luo D, Hou Y, Xu L, Lin D
Journal of neuroinflammation 2021 Sep 20;18(1):216

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Glycogen synthase kinase-3β inhibitor SB216763 promotes DNA repair in ischemic retinal neurons.

Zhang J, Lai ZP, Chen P, Ying Y, Zhuang J, Yu KM
Neural regeneration research 2021 Feb;16(2):394-400

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Crx Is Posttranscriptionally Regulated by Light Stimulation in Postnatal Rat Retina.

Wu Y, Qiu J, Chen S, Chen X, Zhang J, Zhuang J, Liu S, Yang M, Zhou P, Chen H, Ge J, Yu K, Zhuang J
Frontiers in cell and developmental biology 2020;8:174

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Inhibition of microRNA-34a Suppresses Epileptiform Discharges Through Regulating Notch Signaling and Apoptosis in Cultured Hippocampal Neurons.

Wang J, Zheng Y, Cheng X, Xu F, Zhang P, Zhou X, Zhao H
Neurochemical research 2019 May;44(5):1252-1261

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Increased expression of DOC2A in human and rat temporal lobe epilepsy.

Hu X, Tang J, Lan X, Mi X
Epilepsy research 2019 Mar;151:78-84

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Apolipoprotein E Affects In Vitro Axonal Growth and Regeneration via the MAPK Signaling Pathway.

Yin C, Guo ZD, He ZZ, Wang ZY, Sun XC
Cell transplantation 2019 Jun;28(6):691-703

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Minocycline promotes posthemorrhagic neurogenesis via M2 microglia polarization via upregulation of the TrkB/BDNF pathway in rats.

Miao H, Li R, Han C, Lu X, Zhang H
Journal of neurophysiology 2018 Sep 1;120(3):1307-1317

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of MAP2 using anti-MAP2 antibody (A01201-1).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2 antigen affinity purified polyclonal antibody (Catalog # A01201-1) at 0.5 ug/mL overnight at 4

Additional image