MA1-19166

antibody from Invitrogen Antibodies

Targeting: MAP2 MAP2A, MAP2B, MAP2C

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

MA1-19166

Provider

Invitrogen Antibodies

Product name

MAP2 Monoclonal Antibody (MT-01)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse, Bovine, Feline, Porcine

Host

Mouse

Isotype

IgG

Antibody clone number

MT-01

Vial size

100 µg

Concentration

1 mg/mL

Storage

4° C, do not freeze

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of microtubules partially purified from porcine brain lysate. Probed with MAP2 a, b monoclonal antibodies (Product # MA1-19166 (Lane 1)), (Product # MA1-19425 (Lane 2)), (Product # MA1-19426 (Lane 3)).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blotting analysis (reducing conditions) of microtubules partially purified from porcine brain lysate. Lane 1: immunostaining with anti-MAP2ab (MT-01) Monoclonal antibody (Product # MA1-19166); Lane 2: immunostaining with anti-MAP2ab (MT-07); Lane 3: immunostaining with anti-MAP2ab (MT-08).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using MAP2 Monoclonal Antibody (MT-01) (Product # MA1-19166) and a ~270 kDa band corresponding to MAP2 A, B was observed across positive cell lines SH-SY5Y, SH-SY5Y differentiated to neurons (10 µM Retinoic acid for 5 days) and IMR-32 and tissue extract of rat brain. Whole cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), SH-SY5Y treated with Retinoic acid (Lane 2), BJ (Lane 3), BeWo (Lane 4) and IMR-32 (Lane 5); and tissue extract of Rat brain (Lane 6) and Rat brown adipose (Lane 7) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1,000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Microtubule-associated protein 2 was performed using 70% confluent log phase SH-SY5Y cells differentiated to neurons (Retinoic acid 10 uM for 5 days). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MAP2 Monoclonal Antibody (MT-01) (Product # MA1-19166) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing neuronal dendrites, cytoplasm, plasma membrane and cytoskeleton localization. Panel e represents undifferentiated SH-SY5Y cells. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.