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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
- Other assay [1]
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- Product number
- PA5-52605 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Chromogranin B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KQYSSHHTAE KRKRLGELFN PYYDPLQWKS SHFERRDNMN DNFLEGEEEN ELTLNEKNFF PEYNYDWWEK KPFSEDVNWG YEKRNLARVP KLDLKRQYDR VAQLDQLLHY RKKSAEFPDF YDS Highest antigen sequence identity to the following orthologs: Mouse - 73%, Rat - 73%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.08 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references A Drug Repurposing Screen Identifies Fludarabine Phosphate as a Potential Therapeutic Agent for N-MYC Overexpressing Neuroendocrine Prostate Cancers.
Sympathetic signaling facilitates progression of neuroendocrine prostate cancer.
Elhasasna H, Khan R, Bhanumathy KK, Vizeacoumar FS, Walke P, Bautista M, Dahiya DK, Maranda V, Patel H, Balagopal A, Alli N, Krishnan A, Freywald A, Vizeacoumar FJ
Cells 2022 Jul 20;11(14)
Cells 2022 Jul 20;11(14)
Sympathetic signaling facilitates progression of neuroendocrine prostate cancer.
Dwivedi S, Bautista M, Shrestha S, Elhasasna H, Chaphekar T, Vizeacoumar FS, Krishnan A
Cell death discovery 2021 Nov 22;7(1):364
Cell death discovery 2021 Nov 22;7(1):364
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Chromogranin B in Lane 1: Marker (kDa) 250, 130, 100, 70, 55, 35, 25, 15, 10; Lane 2: Human adrenal gland tissue. Samples were probed using a Chromogranin B Polyclonal Antibody (Product # PA5-52605).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Chromogranin B in human cell line SH-SY5Y using a Chromogranin B Polyclonal Antibody (Product # PA5-52605) shows localization to vesicles.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Chromogranin B in human liver using Chromogranin B Polyclonal Antibody (Product # PA5-52605).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Chromogranin B in human kidney using Chromogranin B Polyclonal Antibody (Product # PA5-52605).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Chromogranin B in human adrenal gland and colon tissues using Chromogranin B Polyclonal Antibody (Product # PA5-52605). Corresponding CHGB RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Chromogranin B in human adrenal gland, colon, kidney and liver using Chromogranin B Polyclonal Antibody (Product # PA5-52605) (A) shows similar protein distribution across tissues to an independent Chromogranin B Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Chromogranin B in human adrenal gland using Chromogranin B Polyclonal Antibody (Product # PA5-52605) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Chromogranin B in human colon using Chromogranin B Polyclonal Antibody (Product # PA5-52605) shows low expression as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 NE induces NED markers in PC cells. A Immunostaining shows that 50 uM NE induces the expression of the NED markers CHGB and SYP in DU145 cells at 24 h. Both CHGB and SYP show characteristic granular staining after NE treatment. B 50 uM NE induces the expression of CHGB and SYP in LNCaP cells at 24 h. The control cells display weak basal expression of CHGB and SYP, but NE treatment upregulates the expression of these markers, evident by their increased staining intensity. Scale bar, 20 um.
