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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB6149 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6149, RRID:AB_1578608
- Product name
- PEBP1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of PEBP1.
- Antigen sequence
C-DYVPKLYEQLSGK- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Suppression of Raf-1 kinase activity and MAP kinase signalling by RKIP.
Yeung K, Seitz T, Li S, Janosch P, McFerran B, Kaiser C, Fee F, Katsanakis KD, Rose DW, Mischak H, Sedivy JM, Kolch W
Nature 1999 Sep 9;401(6749):173-7
Nature 1999 Sep 9;401(6749):173-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PEBP1 polyclonal antibody (Cat # PAB6149) staining (0.01 ug/mL) of A-549 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PEBP1 polyclonal antibody (Cat # PAB6149) (1.5 ug/mL) staining of paraffin embedded human cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
