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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NB100-1020 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1020, RRID:AB_2163168
- Product name
- Goat Polyclonal RKIP/PBP Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Suppression of Raf-1 kinase activity and MAP kinase signalling by RKIP.
Yeung K, Seitz T, Li S, Janosch P, McFerran B, Kaiser C, Fee F, Katsanakis KD, Rose DW, Mischak H, Sedivy JM, Kolch W
Nature 1999 Sep 9;401(6749):173-7
Nature 1999 Sep 9;401(6749):173-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RKIP/PBP Antibody [NB100-1020] - (1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RKIP/PBP Antibody [NB100-1020] - Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RKIP/PBP Antibody [NB100-1020] - Staining of paraffin embedded Human Cortex. Antibody at 5 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
