AF4538

antibody from R&D Systems

Targeting: PEBP1 HCNP, PBP, PEBP, RKIP

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF4538

Provider

R&D Systems

Product name

Human/Mouse/Rat RKIP Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects endogenous human, mouse, and rat RKIP in Western blots.

Reactivity

Human, Mouse, Rat

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P30086


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human/Mouse/Rat RKIP by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells and A172 human glioblastoma cell line. PVDF membrane was probed with 0.2 µg/mL of Human/Mouse/Rat RKIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4538) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for RKIP at approximately 23 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.