Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00004924-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004924-M01, RRID:AB_2153657
- Product name
- Mouse Monoclonal Nucleobindin 1 Antibody
- Antibody type
- Monoclonal
- Description
- Ascites. NUCB1 - nucleobindin 1
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nucleobindin 1 Antibody (1H8-2C11) [H00004924-M01] - NUCB1 monoclonal antibody (M01), clone 1H8-2C11 Analysis of NUCB1 expression in HepG2.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Nucleobindin 1 Antibody (1H8-2C11) [H00004924-M01] - Detection limit for recombinant GST tagged NUCB1 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Nucleobindin 1 Antibody (1H8-2C11) [H00004924-M01] - Analysis of monoclonal antibody to NUCB1 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody (1H8-2C11) [H00004924-M01] - Analysis of monoclonal antibody to NUCB1 on formalin-fixed paraffin-embedded human spleen tissue. Antibody concentration 3 ug/ml.