TA503917

antibody from OriGene

Targeting: NUCB1 Calnuc, NUC

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA503917

Provider

OriGene

Proper citation

OriGene Cat#TA503917, RRID:AB_11123830

Product name

NUCB1 (Nucleobindin 1) mouse monoclonal antibody, clone OTI1A3 (formerly 1A3)

Antibody type

Monoclonal

Description

NUCB1 (Nucleobindin 1) mouse monoclonal antibody, clone OTI1A3 (formerly 1A3)

Host

Mouse

Conjugate

Unconjugated

Epitope

NUCB1

Isotype

IgG

Antibody clone number

OTI1A3

Vial size

100 µl

Concentration

0.68 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NUCB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUCB1 (RC201786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUCB1.

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC201786 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NUCB1 antibody(TA503917), and then analyzed by flow cytometry.

Validation comment

FC