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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- TA503930 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA503930, RRID:AB_11128800
- Product name
- NUCB1 (Nucleobindin 1) mouse monoclonal antibody, clone OTI2A5 (formerly 2A5)
- Antibody type
- Monoclonal
- Description
- NUCB1 (Nucleobindin 1) mouse monoclonal antibody, clone OTI2A5 (formerly 2A5)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- NUCB1
- Isotype
- IgG
- Antibody clone number
- OTI2A5
- Vial size
- 100 µl
- Concentration
- 0.65 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUCB1 (RC201786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUCB1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-NUCB1 mouse monoclonal antibody (TA503930) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NUCB1(RC201786).
- Validation comment
- IF
