PA5-30107

antibody from Invitrogen Antibodies

Targeting: NUCB1 Calnuc, NUC

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30107

Provider

Invitrogen Antibodies

Product name

Nucleobindin 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A549, HeLa, HepG2, mouse brain.

Concentration

0.94 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Nucleobindin 1 Polyclonal Antibody (Product # PA5-30107). Sample (30 µg of whole cell lysate). Lane A: Hela. 10% SDS PAGE. Nucleobindin 1 Polyclonal Antibody (Product # PA5-30107) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Nucleobindin 1 Polyclonal Antibody (Product # PA5-30107). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 10% SDS PAGE. Nucleobindin 1 Polyclonal Antibody (Product # PA5-30107) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Nucleobindin 1 in methanol-fixed HeLa cells using a Nucleobindin 1 polyclonal antibody (Product # PA5-30107) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Nucleobindin 1 was performed in U2OS cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Nucleobindin 1 Polyclonal Antibody (Product # PA5 30107) diluted at 1:500. Red: FIS1, a mitochondria marker. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Nucleobindin 1 Polyclonal Antibody detects Nucleobindin 1 protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Nucleobindin 1 Polyclonal Antibody (Product # PA5-30107) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Nucleobindin 1 Polyclonal Antibody detects Nucleobindin 1 protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Nucleobindin 1 Polyclonal Antibody (Product # PA5-30107) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.