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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- HPA008176 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA008176, RRID:AB_1845923
- Product name
- Anti-NUCB1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human NUCB1, Gene description: nucleobindin 1, Alternative Gene Names: Calnuc, NUC, Validated applications: ICC, IHC, WB, Uniprot ID: Q02818, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Nucleobindin-1 regulates ECM degradation by promoting intra-Golgi trafficking of MMPs
Pacheco-Fernandez N, Pakdel M, Blank B, Sanchez-Gonzalez I, Weber K, Tran M, Hecht T, Gautsch R, Beck G, Perez F, Hausser A, Linder S, von Blume J
Journal of Cell Biology 2020;219(8)
Journal of Cell Biology 2020;219(8)
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Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
- Sample type
- Human
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human small intestine and pancreas tissues using HPA008176 antibody. Corresponding NUCB1 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
