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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- MA5-24141 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CADM4 Monoclonal Antibody (404619)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody detects recombinant human (rh) IGSF4C/SynCAM4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) IGSF4A, rhIGSF4B, rhIGSF4D, or rhIGSF8 is observed. Reconstitute at 0.5 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 404619
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of CADM4 in immersion fixed WM- 115 human malignant melanoma cell line (positive staining) and Daudi human Burkitts lymphoma cell line (negative staining). Samples were incubated in CADM4 monoclonal antibody (Product # MA5-24141) using a dilution of 8 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of A172 human glioblastoma cell line was stained with Rat Anti-human IGSF4C/SynCAM4 Monoclonal Antibody (Product # MA5-24141, filled histogram) or isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F (ab')2 Secondary Antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of CADM4 in A172 human glioblastoma cell line. Samples were incubated in CADM4 monoclonal antibody (Product # MA5-24141) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab)2 Secondary Antibody.
