PA5-118201

antibody from Invitrogen Antibodies

Targeting: PSMA2 HC3, MU, PMSA2

Western blot (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118201

Provider

Invitrogen Antibodies

Product name

PSMA2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PSMA2 Immunoprecipitation using: Lane A: 0.5 mg A431 Whole Cell Lysate 4 µL with PSMA2 Polyclonal Antibody (Product # PA5-118201) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: PSMA2 Polyclonal Antibody (Product # PA5-118201), at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 30 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of PSMA2 in Lane A: 0.5 mg A431 Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein A/G beads, incubated with polyclonal antibody (Product # PA5-118201) with a dilution of 1:100 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 26 kDa , Observed band size : 30 kDa .

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) of PSMA2 in human liver. Samples were incubated with polyclonal antibody (Product # PA5-118201) with a dilution of 1:1,000 .

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of PSMA2 in Lane A: 0.5 mg A431 Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein A/G beads, incubated with polyclonal antibody (Product # PA5-118201) with a dilution of 1:100 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 26 kDa , Observed band size : 30 kDa .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PSMA2 Immunoprecipitation using: Lane A: 0.5 mg A431 Whole Cell Lysate 4 µL with PSMA2 Polyclonal Antibody (Product # PA5-118201) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: PSMA2 Polyclonal Antibody (Product # PA5-118201), at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 30 kDa.