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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-47747 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NPDC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-human NPDC-1 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47747) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. Specific bands were detected for NPDC-1 at approximately 40 kDa and 54 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NPDC1 in human brain tissue. Samples were incubated in NPDC1 polyclonal antibody (Product # PA5-47747) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. Specific bands were detected for NPDC‚1 at approximately 40 kDa and 54 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NPDC1 in immersion fixed paraffin-embedded sections of human brain (hippocampus). Samples were incubated in NPDC1 polyclonal antibody (Product # PA5-47747) using a dilution of 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic . Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and synaptic vesicles of neuronal processes.
