H00002771-M02

antibody from Abnova Corporation

Targeting: GNAI2 GIP, GNAI2B

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002771-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002771-M02, RRID:AB_581846

Product name

GNAI2 monoclonal antibody (M02), clone 3G11

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant GNAI2.

Antigen sequence

MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVK
LLLLGAGESGKSTIVKQMKIIHEDGYSEEECRQYR
AVVYSNTIQSIMAIVKAMGNLQIDFADPSRADDAR
QLFALSCTAEEQGVLPDDLSGVIRRLWADHGVQAC
FGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDV
LRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERK
KWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMH
ESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIT
HSPLTICFPEYTGANKYDEAASYIQSKFEDLNKRK
DTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLK
DCGLF


Isotype

IgG

Antibody clone number

3G11

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

GNAI2 monoclonal antibody (M02), clone 3G11. Western Blot analysis of GNAI2 expression in MCF-7 ( Cat # L046V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged GNAI2 is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between C5AR1 and GNAI2. HeLa cells were stained with anti-C5AR1 rabbit purified polyclonal 1:1200 and anti-GNAI2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)