PA5-52970

antibody from Invitrogen Antibodies

Targeting: SPTBN1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-52970

Provider

Invitrogen Antibodies

Product name

SPTBN1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: PLATSTDHGH NLQTVQLLIK KNQTLQKEIQ GHQPRIDDIF ERSQNIVTDS SSLSAEAIRQ RLADLKQLWG LLIEETEKRH RRLEEAHRAQ QYYFDAAEAE AWMSEQEL

Concentration

0.1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Spectrin beta-1 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue. Samples were probed using a Spectrin beta-1 Polyclonal Antibody (Product # PA5-52970).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Spectrin beta-1 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a Spectrin beta-1 Polyclonal Antibody (Product # PA5-52970).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of Spectrin beta-1 in human cell line A-431 using a Spectrin beta-1 Polyclonal Antibody (Product # PA5-52970) shows localization to the Golgi apparatus.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Spectrin beta-1 in human lung and pancreas tissues using Spectrin beta-1 Polyclonal Antibody (Product # PA5-52970). Corresponding SPTBN1 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Spectrin beta-1 in human cerebral cortex using Spectrin beta-1 Polyclonal Antibody (Product # PA5-52970) shows moderate to strong positivity in neuropil.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Spectrin beta-1 in human lung using Spectrin beta-1 Polyclonal Antibody (Product # PA5-52970) shows weak to moderate positivity in respiratory epithelial cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Spectrin beta-1 in human kidney using Spectrin beta-1 Polyclonal Antibody (Product # PA5-52970) shows weak to moderate membranous positivity in cells in tubules.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Spectrin beta-1 in human pancreas using Spectrin beta-1 Polyclonal Antibody (Product # PA5-52970) shows weak to moderate membranous positivity in exocrine glandular cells.