Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP1-82964 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-82964, RRID:AB_11035437
- Product name
- Rabbit Polyclonal SV2A Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human SV2A antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Amyloid beta a4 precursor protein-binding family B member 1 (FE65) interactomics revealed synaptic vesicle glycoprotein 2A (SV2A) and sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (SERCA2) as new binding proteins in the human brain.
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Nensa FM, Neumann MH, Schrötter A, Przyborski A, Mastalski T, Susdalzew S, Looβe C, Helling S, El Magraoui F, Erdmann R, Meyer HE, Uszkoreit J, Eisenacher M, Suh J, Guénette SY, Röhner N, Kögel D, Theiss C, Marcus K, Müller T
Molecular & cellular proteomics : MCP 2014 Feb;13(2):475-88
Molecular & cellular proteomics : MCP 2014 Feb;13(2):475-88
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23
Nature methods 2013 Apr;10(4):315-23
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SV2A Antibody [NBP1-82964] - Analysis of SV2A in (1) undifferentiated SH-SY5Y, (2) differentiated SH-SY5Y (neurons) and (3) mouse brain using anti-SV2A antibody. 30ug of protein/lane. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SV2A Antibody [NBP1-82964] - Analysis in human cell lines U2OS and PC-3. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SV2A Antibody [NBP1-82964] - Analysis in human cerebral cortex tissue.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SV2A Antibody [NBP1-82964] - Staining of neuroblastoma cells SH-SY5Y differentiated into neurons. Lane (1) 10ug of input, 500ug used for IP. 10ul of antibody used for incubation with lysate (overnight incubation at 4C with rotation) prior to addition of beads. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964] - Analysis in human cerebral cortex and small intestine tissues using HPA007863 antibody. Corresponding SV2A RNA-seq dat
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964] - Staining of human cerebellum shows strong cytoplasmic positivity in neuropil.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964] - Staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964] - Staining of human small intestine shows no positivity in glandular cells.