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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Product number
- PA5-40120 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KDM5D Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: Jurkat and human cerebellar tissue.
- Concentration
- 1 mg/mL
Submitted references Imidazopyridines as Potent KDM5 Demethylase Inhibitors Promoting Reprogramming Efficiency of Human iPSCs.
Dabiri Y, Gama-Brambila RA, Taškova K, Herold K, Reuter S, Adjaye J, Utikal J, Mrowka R, Wang J, Andrade-Navarro MA, Cheng X
iScience 2019 Feb 22;12:168-181
iScience 2019 Feb 22;12:168-181
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KDM5D in human tissue extracts (30 µg). Samples were separated by 5 % SDS-PAGE and the membrane was probed with a KDM5D polyclonal antibody (Product # PA5-40120) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KDM5D in various whole cell extracts (30 µg). Samples were separated by 5% SDS-PAGE and the membrane was probed with a KDM5D polyclonal antibody (Product # PA5-40120) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM5D antibody (Product # PA5-40120) diluted at 1:1000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of KDM5D was performed by separating 30 µg of Jurkat whole cell and nuclear extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a KDM5D Polyclonal Antibody (Product # PA5-40120) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
