MA5-27639

antibody from Invitrogen Antibodies

Targeting: BCAN BEHAB, CSPG7, MGC13038

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27639

Provider

Invitrogen Antibodies

Product name

Brevican Monoclonal Antibody (S294A-6)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

S294A-6

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Brevican in rat brain membrane with 10 µg of sample. The sample was incubated with Brevican monoclonal antibody (Product # MA5-27639) using a dilution of 1:1000 (1 hour at RT), and followed by Goat Anti-Mouse (1 hour at RT) at a dilution of 1:200 and TMB development (10 min at RT). Samples were arranged as follows: Lane 1: MW Ladder, Lane 2: Rat Brain membrane.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Brevican in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with Brevican monoclonal antibody (Product # MA5-27639) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) Brevican Antibody, D) Merged image. Magnification: 60x.