H00001617-M01

antibody from Abnova Corporation

Targeting: DAZ1 DAZ, SPGY

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001617-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001617-M01, RRID:AB_534832

Product name

DAZ1 monoclonal antibody (M01), clone 3G10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant DAZ1.

Antigen sequence

SSSAAASQGWVLPEGKIVPNTVFVGGIDARMDETE
IGSCFGRYGSVKEVKIITNRTGVSKGYGFVSFVND
VDVQKIVGSQIHFHGKKLKLGPAIRKQKLC


Isotype

IgG

Antibody clone number

3G10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

DAZ1 monoclonal antibody (M01), clone 3G10. Western Blot analysis of DAZ1 expression in human pancreas.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged DAZ1 is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to DAZ1 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to DAZ1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol