Antibody data
- Antibody Data
- Antigen structure
- References [27]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- 10894-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#10894-1-AP, RRID:AB_2292715
- Product name
- CYB5R3 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated CYB5R3 antibody (Cat. #10894-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, pig, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Divergent role of Mitochondrial Amidoxime Reducing Component 1 (MARC1) in human and mouse.
Oxidized soluble guanylyl cyclase causes erectile dysfunction in alcoholic mice.
LINC00116-encoded microprotein mitoregulin regulates fatty acid metabolism at the mitochondrial outer membrane.
Sex-specific metabolic adaptations in transgenic mice overexpressing cytochrome b(5) reductase-3.
CYB5R3 in type II alveolar epithelial cells protects against lung fibrosis by suppressing TGF-β1 signaling.
Cyb5r3-based mechanism and reversal of secondary failure to sulfonylurea in diabetes.
Hydrogen sulfide regulates the redox state of soluble guanylate cyclase in CSE(-/-) mice corpus cavernosum microcirculation.
Cigarette smoke induces pulmonary arterial dysfunction through an imbalance in the redox status of the soluble guanylyl cyclase.
Smooth muscle cell CYB5R3 preserves cardiac and vascular function under chronic hypoxic stress.
Role of cytoglobin in cigarette smoke constituent-induced loss of nitric oxide bioavailability in vascular smooth muscle cells.
The UFM1 system regulates ER-phagy through the ufmylation of CYB5R3.
CYB5R3 overexpression preserves skeletal muscle mitochondria and autophagic signaling in aged transgenic mice.
Identification and Validation of a Nine-Gene Amino Acid Metabolism-Related Risk Signature in HCC.
GGPP depletion initiates metaflammation through disequilibrating CYB5R3-dependent eicosanoid metabolism.
Mitochondrial adaptations in liver and skeletal muscle to pro-longevity nutritional and genetic interventions: the crosstalk between calorie restriction and CYB5R3 overexpression in transgenic mice.
Loss of smooth muscle CYB5R3 amplifies angiotensin II-induced hypertension by increasing sGC heme oxidation.
Conditional deletion of smooth muscle Cullin-3 causes severe progressive hypertension.
Smooth muscle cytochrome b5 reductase 3 deficiency accelerates pulmonary hypertension development in sickle cell mice.
Dia1-dependent adhesions are required by epithelial tissues to initiate invasion.
High expression of cytochrome b 5 reductase isoform 3/cytochrome b 5 system in the cerebellum and pyramidal neurons of adult rat brain.
Structure Guided Chemical Modifications of Propylthiouracil Reveal Novel Small Molecule Inhibitors of Cytochrome b5 Reductase 3 That Increase Nitric Oxide Bioavailability.
Purified NADH-cytochrome b5 reductase is a novel superoxide anion source inhibited by apocynin: sensitivity to nitric oxide and peroxynitrite.
Caveolin-rich lipid rafts of the plasma membrane of mature cerebellar granule neurons are microcompartments for calcium/reactive oxygen and nitrogen species cross-talk signaling.
Stimulation and clustering of cytochrome b5 reductase in caveolin-rich lipid microdomains is an early event in oxidative stress-mediated apoptosis of cerebellar granule neurons.
L-type calcium channels and cytochrome b5 reductase are components of protein complexes tightly associated with lipid rafts microdomains of the neuronal plasma membrane.
Clustering of plasma membrane-bound cytochrome b5 reductase within 'lipid raft' microdomains of the neuronal plasma membrane.
Neuronal expression and subcellular localization of cholesterol 24-hydroxylase in the mouse brain.
Smagris E, Shihanian LM, Mintah IJ, Bigdelou P, Livson Y, Brown H, Verweij N, Hunt C, Johnson RO, Greer TJ, Hartford SA, Hindy G, Sun L, Nielsen JB, Halasz G, Lotta LA, Murphy AJ, Sleeman MW, Gusarova V
PLoS genetics 2024 Mar;20(3):e1011179
PLoS genetics 2024 Mar;20(3):e1011179
Oxidized soluble guanylyl cyclase causes erectile dysfunction in alcoholic mice.
Olivencia MA, Gil de Biedma-Elduayen L, Giménez-Gómez P, Barreira B, Fernández A, Angulo J, Colado MI, O'Shea E, Perez-Vizcaino F
British journal of pharmacology 2023 Sep;180(18):2361-2376
British journal of pharmacology 2023 Sep;180(18):2361-2376
LINC00116-encoded microprotein mitoregulin regulates fatty acid metabolism at the mitochondrial outer membrane.
Zhang S, Guo Y, Fidelito G, Robinson DRL, Liang C, Lim R, Bichler Z, Guo R, Wu G, Xu H, Zhou QD, Singh BK, Yen P, Kappei D, Stroud DA, Ho L
iScience 2023 Sep 15;26(9):107558
iScience 2023 Sep 15;26(9):107558
Sex-specific metabolic adaptations in transgenic mice overexpressing cytochrome b(5) reductase-3.
Sánchez-Mendoza LM, Pérez-Sánchez C, Rodríguez-López S, López-Pedrera C, Calvo-Rubio M, de Cabo R, Burón MI, González-Reyes JA, Villalba JM
Free radical biology & medicine 2023 Oct;207:144-160
Free radical biology & medicine 2023 Oct;207:144-160
CYB5R3 in type II alveolar epithelial cells protects against lung fibrosis by suppressing TGF-β1 signaling.
Bueno M, Calyeca J, Khaliullin T, Miller MP, Alvarez D, Rosas L, Brands J, Baker C, Nasser A, Shulkowski S, Mathien A, Uzoukwu N, Sembrat J, Mays BG, Fiedler K, Hahn SA, Salvatore SR, Schopfer FJ, Rojas M, Sandner P, Straub AC, Mora AL
JCI insight 2023 Mar 8;8(5)
JCI insight 2023 Mar 8;8(5)
Cyb5r3-based mechanism and reversal of secondary failure to sulfonylurea in diabetes.
Watanabe H, Du W, Son J, Sui L, Asahara SI, Kurland IJ, Kuo T, Kitamoto T, Miyachi Y, de Cabo R, Accili D
Science translational medicine 2023 Feb;15(681):eabq4126
Science translational medicine 2023 Feb;15(681):eabq4126
Hydrogen sulfide regulates the redox state of soluble guanylate cyclase in CSE(-/-) mice corpus cavernosum microcirculation.
Olivencia MA, Esposito E, Brancaleone V, Castaldo S, Cirino G, Pérez-Vizcaino F, Sorrentino R, d'Emmanuele di Villa Bianca R, Mitidieri E
Pharmacological research 2023 Aug;194:106834
Pharmacological research 2023 Aug;194:106834
Cigarette smoke induces pulmonary arterial dysfunction through an imbalance in the redox status of the soluble guanylyl cyclase.
Sevilla-Montero J, Munar-Rubert O, Pino-Fadón J, Aguilar-Latorre C, Villegas-Esguevillas M, Climent B, Agrò M, Choya-Foces C, Martínez-Ruiz A, Balsa E, Muñoz-Calleja C, Gómez-Punter RM, Vázquez-Espinosa E, Cogolludo A, Calzada MJ
Free radical biology & medicine 2022 Nov 20;193(Pt 1):9-22
Free radical biology & medicine 2022 Nov 20;193(Pt 1):9-22
Smooth muscle cell CYB5R3 preserves cardiac and vascular function under chronic hypoxic stress.
Durgin BG, Wood KC, Hahn SA, McMahon B, Baust JJ, Straub AC
Journal of molecular and cellular cardiology 2022 Jan;162:72-80
Journal of molecular and cellular cardiology 2022 Jan;162:72-80
Role of cytoglobin in cigarette smoke constituent-induced loss of nitric oxide bioavailability in vascular smooth muscle cells.
Mahgoup EM, Khaleel SA, El-Mahdy MA, Abd-Allah AR, Zweier JL
Nitric oxide : biology and chemistry 2022 Feb 1;119:9-18
Nitric oxide : biology and chemistry 2022 Feb 1;119:9-18
The UFM1 system regulates ER-phagy through the ufmylation of CYB5R3.
Ishimura R, El-Gowily AH, Noshiro D, Komatsu-Hirota S, Ono Y, Shindo M, Hatta T, Abe M, Uemura T, Lee-Okada HC, Mohamed TM, Yokomizo T, Ueno T, Sakimura K, Natsume T, Sorimachi H, Inada T, Waguri S, Noda NN, Komatsu M
Nature communications 2022 Dec 21;13(1):7857
Nature communications 2022 Dec 21;13(1):7857
CYB5R3 overexpression preserves skeletal muscle mitochondria and autophagic signaling in aged transgenic mice.
López-Bellón S, Rodríguez-López S, González-Reyes JA, Burón MI, de Cabo R, Villalba JM
GeroScience 2022 Aug;44(4):2223-2241
GeroScience 2022 Aug;44(4):2223-2241
Identification and Validation of a Nine-Gene Amino Acid Metabolism-Related Risk Signature in HCC.
Zhao Y, Zhang J, Wang S, Jiang Q, Xu K
Frontiers in cell and developmental biology 2021;9:731790
Frontiers in cell and developmental biology 2021;9:731790
GGPP depletion initiates metaflammation through disequilibrating CYB5R3-dependent eicosanoid metabolism.
Wei L, Zheng YY, Sun J, Wang P, Tao T, Li Y, Chen X, Sang Y, Chong D, Zhao W, Zhou Y, Wang Y, Jiang Z, Qiu T, Li CJ, Zhu MS, Zhang X
The Journal of biological chemistry 2020 Nov 20;295(47):15988-16001
The Journal of biological chemistry 2020 Nov 20;295(47):15988-16001
Mitochondrial adaptations in liver and skeletal muscle to pro-longevity nutritional and genetic interventions: the crosstalk between calorie restriction and CYB5R3 overexpression in transgenic mice.
Rodríguez-López S, López-Bellón S, González-Reyes JA, Burón MI, de Cabo R, Villalba JM
GeroScience 2020 Jun;42(3):977-994
GeroScience 2020 Jun;42(3):977-994
Loss of smooth muscle CYB5R3 amplifies angiotensin II-induced hypertension by increasing sGC heme oxidation.
Durgin BG, Hahn SA, Schmidt HM, Miller MP, Hafeez N, Mathar I, Freitag D, Sandner P, Straub AC
JCI insight 2019 Oct 3;4(19)
JCI insight 2019 Oct 3;4(19)
Conditional deletion of smooth muscle Cullin-3 causes severe progressive hypertension.
Agbor LN, Nair AR, Wu J, Lu KT, Davis DR, Keen HL, Quelle FW, McCormick JA, Singer JD, Sigmund CD
JCI insight 2019 Jun 11;5(14)
JCI insight 2019 Jun 11;5(14)
Smooth muscle cytochrome b5 reductase 3 deficiency accelerates pulmonary hypertension development in sickle cell mice.
Wood KC, Durgin BG, Schmidt HM, Hahn SA, Baust JJ, Bachman T, Vitturi DA, Ghosh S, Ofori-Acquah SF, Mora AL, Gladwin MT, Straub AC
Blood advances 2019 Dec 10;3(23):4104-4116
Blood advances 2019 Dec 10;3(23):4104-4116
Dia1-dependent adhesions are required by epithelial tissues to initiate invasion.
Fessenden TB, Beckham Y, Perez-Neut M, Ramirez-San Juan G, Chourasia AH, Macleod KF, Oakes PW, Gardel ML
The Journal of cell biology 2018 Apr 2;217(4):1485-1502
The Journal of cell biology 2018 Apr 2;217(4):1485-1502
High expression of cytochrome b 5 reductase isoform 3/cytochrome b 5 system in the cerebellum and pyramidal neurons of adult rat brain.
Samhan-Arias AK, López-Sánchez C, Marques-da-Silva D, Lagoa R, Garcia-Lopez V, García-Martínez V, Gutierrez-Merino C
Brain structure & function 2016 May;221(4):2147-62
Brain structure & function 2016 May;221(4):2147-62
Structure Guided Chemical Modifications of Propylthiouracil Reveal Novel Small Molecule Inhibitors of Cytochrome b5 Reductase 3 That Increase Nitric Oxide Bioavailability.
Rahaman MM, Reinders FG, Koes D, Nguyen AT, Mutchler SM, Sparacino-Watkins C, Alvarez RA, Miller MP, Cheng D, Chen BB, Jackson EK, Camacho CJ, Straub AC
The Journal of biological chemistry 2015 Jul 3;290(27):16861-72
The Journal of biological chemistry 2015 Jul 3;290(27):16861-72
Purified NADH-cytochrome b5 reductase is a novel superoxide anion source inhibited by apocynin: sensitivity to nitric oxide and peroxynitrite.
Samhan-Arias AK, Gutierrez-Merino C
Free radical biology & medicine 2014 Aug;73:174-89
Free radical biology & medicine 2014 Aug;73:174-89
Caveolin-rich lipid rafts of the plasma membrane of mature cerebellar granule neurons are microcompartments for calcium/reactive oxygen and nitrogen species cross-talk signaling.
Marques-da-Silva D, Gutierrez-Merino C
Cell calcium 2014 Aug;56(2):108-23
Cell calcium 2014 Aug;56(2):108-23
Stimulation and clustering of cytochrome b5 reductase in caveolin-rich lipid microdomains is an early event in oxidative stress-mediated apoptosis of cerebellar granule neurons.
Samhan-Arias AK, Marques-da-Silva D, Yanamala N, Gutierrez-Merino C
Journal of proteomics 2012 Jun 6;75(10):2934-49
Journal of proteomics 2012 Jun 6;75(10):2934-49
L-type calcium channels and cytochrome b5 reductase are components of protein complexes tightly associated with lipid rafts microdomains of the neuronal plasma membrane.
Marques-da-Silva D, Samhan-Arias AK, Tiago T, Gutierrez-Merino C
Journal of proteomics 2010 Jun 16;73(8):1502-10
Journal of proteomics 2010 Jun 16;73(8):1502-10
Clustering of plasma membrane-bound cytochrome b5 reductase within 'lipid raft' microdomains of the neuronal plasma membrane.
Samhan-Arias AK, Garcia-Bereguiain MA, Martin-Romero FJ, Gutierrez-Merino C
Molecular and cellular neurosciences 2009 Jan;40(1):14-26
Molecular and cellular neurosciences 2009 Jan;40(1):14-26
Neuronal expression and subcellular localization of cholesterol 24-hydroxylase in the mouse brain.
Ramirez DM, Andersson S, Russell DW
The Journal of comparative neurology 2008 Apr 10;507(5):1676-93
The Journal of comparative neurology 2008 Apr 10;507(5):1676-93
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 10894-1-AP(CYB5R3 antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using CYB5R3 antibody 10894-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-CYB5R3 (IP:10894-1-AP, 3ug; Detection:10894-1-AP 1:1000) with HepG2 cells lysate 6000ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human lung cancer using 10894-1-AP(CYB5R3 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue