10894-1-AP

antibody from Proteintech Group

Targeting: CYB5R3 DIA1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

10894-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#10894-1-AP, RRID:AB_2292715

Product name

CYB5R3 antibody

Antibody type

Polyclonal

Description

KD/KO validated CYB5R3 antibody (Cat. #10894-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, pig, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat, Porcine

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Mitoregulin supports mitochondrial membrane integrity and protects against cardiac ischaemia-reperfusion injury.

Stein CS, Zhang X, Witmer NH, Pennington ER, Hahn S, Straub AC, Shaikh SR, Boudreau RL
Cardiovascular research 2026 Mar 26;122(3):379-396

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Beta arrestin 1 is a key regulator of pulmonary vascular tone.

Lebender LF, Seidinger A, Matthey M, Dyck B, Schlamm C, Kaddoura A, Hausherr M, Eggers B, Marcus K, Kostenis E, Gieselmann V, Adamzik M, Klinke A, Koos B, Fleischmann BK, Wenzel D
Proceedings of the National Academy of Sciences of the United States of America 2026 Feb 17;123(7):e2512602123

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The secreted metabolite sensor CtBP2 links metabolism to healthy lifespan.

Sekiya M, Kainoh K, Chen W, Yamazaki D, Tsuyuzaki T, Kobari Y, Nakata A, Saito K, Aono-Soma N, Majid A, Ohno H, Miyamoto T, Matsuzaka T, Nakajima R, Matsuda T, Murayama Y, Sugano Y, Osaki Y, Iwasaki H, Shimano H
Nature aging 2025 Nov;5(11):2197-2214

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The Epstein-Barr virus deubiquitinase BPLF1 regulates stress-induced ribosome UFMylation and reticulophagy.

Liu J, Nagy N, Ayala-Torres C, Bleuse S, Aguilar-Alonso F, Larsson O, Masucci MG
Autophagy 2025 May;21(5):996-1018

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Sex-dependent adaptations in heart mitochondria from transgenic mice overexpressing cytochrome b(5) reductase-3.

Sánchez-Mendoza LM, González-Reyes JA, Rodríguez-López S, Calvo-Rubio M, Calero-Rodríguez P, de Cabo R, Burón MI, Villalba JM
Mitochondrion 2025 Mar;81:102004

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β-Cell Dedifferentiation in HOMA-βlow and HOMA-βhigh Subjects.

Kang F, Zhang Z, Fu H, Sun J, Zhang J, Wang Q
The Journal of clinical endocrinology and metabolism 2025 Apr 22;110(5):e1430-e1438

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CYB5R3 overexpression exhibits sexual dimorphism: Mitochondrial and metabolic adaptations in transgenic female mice during calorie restriction.

Sánchez-Mendoza LM, Pérez-Sánchez C, García-Caballero C, Pérez-Rodríguez M, Calero-Rodríguez P, Vellón-García B, Moreno JA, Burón MI, de Cabo R, González-Reyes JA, Villalba JM
Free radical biology & medicine 2024 Oct;223:69-86

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Divergent role of Mitochondrial Amidoxime Reducing Component 1 (MARC1) in human and mouse.

Smagris E, Shihanian LM, Mintah IJ, Bigdelou P, Livson Y, Brown H, Verweij N, Hunt C, Johnson RO, Greer TJ, Hartford SA, Hindy G, Sun L, Nielsen JB, Halasz G, Lotta LA, Murphy AJ, Sleeman MW, Gusarova V
PLoS genetics 2024 Mar;20(3):e1011179

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Apolipoprotein O modulates cholesterol metabolism via NRF2/CYB5R3 independent of LDL receptor.

Chen J, Hu J, Guo X, Yang Y, Qin D, Tang X, Huang Z, Wang F, Hu D, Peng D, Yu B
Cell death & disease 2024 Jun 3;15(6):389

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Pancreatic β cell interleukin-22 receptor subunit alpha 1 deficiency impairs β cell function in type 2 diabetes via cytochrome b5 reductase 3.

Yu F, Xie S, Wang T, Huang Y, Zhang H, Peng D, Feng Y, Yang Y, Zhang Z, Zhu Y, Meng Z, Zhang R, Li X, Yin H, Xu J, Hu C
Cell reports 2024 Dec 24;43(12):115057

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Oxidized soluble guanylyl cyclase causes erectile dysfunction in alcoholic mice.

Olivencia MA, Gil de Biedma-Elduayen L, Giménez-Gómez P, Barreira B, Fernández A, Angulo J, Colado MI, O'Shea E, Perez-Vizcaino F
British journal of pharmacology 2023 Sep;180(18):2361-2376

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LINC00116-encoded microprotein mitoregulin regulates fatty acid metabolism at the mitochondrial outer membrane.

Zhang S, Guo Y, Fidelito G, Robinson DRL, Liang C, Lim R, Bichler Z, Guo R, Wu G, Xu H, Zhou QD, Singh BK, Yen P, Kappei D, Stroud DA, Ho L
iScience 2023 Sep 15;26(9):107558

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Sex-specific metabolic adaptations in transgenic mice overexpressing cytochrome b(5) reductase-3.

Sánchez-Mendoza LM, Pérez-Sánchez C, Rodríguez-López S, López-Pedrera C, Calvo-Rubio M, de Cabo R, Burón MI, González-Reyes JA, Villalba JM
Free radical biology & medicine 2023 Oct;207:144-160

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Resveratrol reduces ROS-induced ferroptosis by activating SIRT3 and compensating the GSH/GPX4 pathway.

Wang X, Shen T, Lian J, Deng K, Qu C, Li E, Li G, Ren Y, Wang Z, Jiang Z, Sun X, Li X
Molecular medicine (Cambridge, Mass.) 2023 Oct 19;29(1):137

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CYB5R3 in type II alveolar epithelial cells protects against lung fibrosis by suppressing TGF-β1 signaling.

Bueno M, Calyeca J, Khaliullin T, Miller MP, Alvarez D, Rosas L, Brands J, Baker C, Nasser A, Shulkowski S, Mathien A, Uzoukwu N, Sembrat J, Mays BG, Fiedler K, Hahn SA, Salvatore SR, Schopfer FJ, Rojas M, Sandner P, Straub AC, Mora AL
JCI insight 2023 Mar 8;8(5)

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Cyb5r3-based mechanism and reversal of secondary failure to sulfonylurea in diabetes.

Watanabe H, Du W, Son J, Sui L, Asahara SI, Kurland IJ, Kuo T, Kitamoto T, Miyachi Y, de Cabo R, Accili D
Science translational medicine 2023 Feb;15(681):eabq4126

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Hydrogen sulfide regulates the redox state of soluble guanylate cyclase in CSE(-/-) mice corpus cavernosum microcirculation.

Olivencia MA, Esposito E, Brancaleone V, Castaldo S, Cirino G, Pérez-Vizcaino F, Sorrentino R, d'Emmanuele di Villa Bianca R, Mitidieri E
Pharmacological research 2023 Aug;194:106834

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Cigarette smoke induces pulmonary arterial dysfunction through an imbalance in the redox status of the soluble guanylyl cyclase.

Sevilla-Montero J, Munar-Rubert O, Pino-Fadón J, Aguilar-Latorre C, Villegas-Esguevillas M, Climent B, Agrò M, Choya-Foces C, Martínez-Ruiz A, Balsa E, Muñoz-Calleja C, Gómez-Punter RM, Vázquez-Espinosa E, Cogolludo A, Calzada MJ
Free radical biology & medicine 2022 Nov 20;193(Pt 1):9-22

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Smooth muscle cell CYB5R3 preserves cardiac and vascular function under chronic hypoxic stress.

Durgin BG, Wood KC, Hahn SA, McMahon B, Baust JJ, Straub AC
Journal of molecular and cellular cardiology 2022 Jan;162:72-80

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Role of cytoglobin in cigarette smoke constituent-induced loss of nitric oxide bioavailability in vascular smooth muscle cells.

Mahgoup EM, Khaleel SA, El-Mahdy MA, Abd-Allah AR, Zweier JL
Nitric oxide : biology and chemistry 2022 Feb 1;119:9-18

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The UFM1 system regulates ER-phagy through the ufmylation of CYB5R3.

Ishimura R, El-Gowily AH, Noshiro D, Komatsu-Hirota S, Ono Y, Shindo M, Hatta T, Abe M, Uemura T, Lee-Okada HC, Mohamed TM, Yokomizo T, Ueno T, Sakimura K, Natsume T, Sorimachi H, Inada T, Waguri S, Noda NN, Komatsu M
Nature communications 2022 Dec 21;13(1):7857

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CYB5R3 overexpression preserves skeletal muscle mitochondria and autophagic signaling in aged transgenic mice.

López-Bellón S, Rodríguez-López S, González-Reyes JA, Burón MI, de Cabo R, Villalba JM
GeroScience 2022 Aug;44(4):2223-2241

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Identification and Validation of a Nine-Gene Amino Acid Metabolism-Related Risk Signature in HCC.

Zhao Y, Zhang J, Wang S, Jiang Q, Xu K
Frontiers in cell and developmental biology 2021;9:731790

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GGPP depletion initiates metaflammation through disequilibrating CYB5R3-dependent eicosanoid metabolism.

Wei L, Zheng YY, Sun J, Wang P, Tao T, Li Y, Chen X, Sang Y, Chong D, Zhao W, Zhou Y, Wang Y, Jiang Z, Qiu T, Li CJ, Zhu MS, Zhang X
The Journal of biological chemistry 2020 Nov 20;295(47):15988-16001

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Mitochondrial adaptations in liver and skeletal muscle to pro-longevity nutritional and genetic interventions: the crosstalk between calorie restriction and CYB5R3 overexpression in transgenic mice.

Rodríguez-López S, López-Bellón S, González-Reyes JA, Burón MI, de Cabo R, Villalba JM
GeroScience 2020 Jun;42(3):977-994

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Loss of smooth muscle CYB5R3 amplifies angiotensin II-induced hypertension by increasing sGC heme oxidation.

Durgin BG, Hahn SA, Schmidt HM, Miller MP, Hafeez N, Mathar I, Freitag D, Sandner P, Straub AC
JCI insight 2019 Oct 3;4(19)

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Conditional deletion of smooth muscle Cullin-3 causes severe progressive hypertension.

Agbor LN, Nair AR, Wu J, Lu KT, Davis DR, Keen HL, Quelle FW, McCormick JA, Singer JD, Sigmund CD
JCI insight 2019 Jun 11;5(14)

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Smooth muscle cytochrome b5 reductase 3 deficiency accelerates pulmonary hypertension development in sickle cell mice.

Wood KC, Durgin BG, Schmidt HM, Hahn SA, Baust JJ, Bachman T, Vitturi DA, Ghosh S, Ofori-Acquah SF, Mora AL, Gladwin MT, Straub AC
Blood advances 2019 Dec 10;3(23):4104-4116

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Dia1-dependent adhesions are required by epithelial tissues to initiate invasion.

Fessenden TB, Beckham Y, Perez-Neut M, Ramirez-San Juan G, Chourasia AH, Macleod KF, Oakes PW, Gardel ML
The Journal of cell biology 2018 Apr 2;217(4):1485-1502

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High expression of cytochrome b 5 reductase isoform 3/cytochrome b 5 system in the cerebellum and pyramidal neurons of adult rat brain.

Samhan-Arias AK, López-Sánchez C, Marques-da-Silva D, Lagoa R, Garcia-Lopez V, García-Martínez V, Gutierrez-Merino C
Brain structure & function 2016 May;221(4):2147-62

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Structure Guided Chemical Modifications of Propylthiouracil Reveal Novel Small Molecule Inhibitors of Cytochrome b5 Reductase 3 That Increase Nitric Oxide Bioavailability.

Rahaman MM, Reinders FG, Koes D, Nguyen AT, Mutchler SM, Sparacino-Watkins C, Alvarez RA, Miller MP, Cheng D, Chen BB, Jackson EK, Camacho CJ, Straub AC
The Journal of biological chemistry 2015 Jul 3;290(27):16861-72

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Purified NADH-cytochrome b5 reductase is a novel superoxide anion source inhibited by apocynin: sensitivity to nitric oxide and peroxynitrite.

Samhan-Arias AK, Gutierrez-Merino C
Free radical biology & medicine 2014 Aug;73:174-89

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Caveolin-rich lipid rafts of the plasma membrane of mature cerebellar granule neurons are microcompartments for calcium/reactive oxygen and nitrogen species cross-talk signaling.

Marques-da-Silva D, Gutierrez-Merino C
Cell calcium 2014 Aug;56(2):108-23

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Stimulation and clustering of cytochrome b5 reductase in caveolin-rich lipid microdomains is an early event in oxidative stress-mediated apoptosis of cerebellar granule neurons.

Samhan-Arias AK, Marques-da-Silva D, Yanamala N, Gutierrez-Merino C
Journal of proteomics 2012 Jun 6;75(10):2934-49

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L-type calcium channels and cytochrome b5 reductase are components of protein complexes tightly associated with lipid rafts microdomains of the neuronal plasma membrane.

Marques-da-Silva D, Samhan-Arias AK, Tiago T, Gutierrez-Merino C
Journal of proteomics 2010 Jun 16;73(8):1502-10

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Clustering of plasma membrane-bound cytochrome b5 reductase within 'lipid raft' microdomains of the neuronal plasma membrane.

Samhan-Arias AK, Garcia-Bereguiain MA, Martin-Romero FJ, Gutierrez-Merino C
Molecular and cellular neurosciences 2009 Jan;40(1):14-26

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Neuronal expression and subcellular localization of cholesterol 24-hydroxylase in the mouse brain.

Ramirez DM, Andersson S, Russell DW
The Journal of comparative neurology 2008 Apr 10;507(5):1676-93

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 10894-1-AP(CYB5R3 antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using CYB5R3 antibody 10894-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-CYB5R3 (IP:10894-1-AP, 3ug; Detection:10894-1-AP 1:1000) with HepG2 cells lysate 6000ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human lung cancer using 10894-1-AP(CYB5R3 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue