Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- CF501385 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYB5R3 Monoclonal Antibody (OTI1E3), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI1E3
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYB5R3 (RC201592, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-CYB5R. Positive lysates LY400140 (100 µg) and LC400140 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-CYB5R3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). (1:200)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-CYB5R3 mouse monoclonal antibody (TA501385) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CYB5R3(RC201592).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-CYB5R3 mouse monoclonal antibody (TA501385) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CYB5R3(RC201592).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human thyroid tissue within the normal limits using anti-CYB5R3 mouse monoclonal antibody. (TA501385)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC201592 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CYB5R3 antibody (TA501385), and then analyzed by flow cytometry.
