Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [2]
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Validation data
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- Product number
- LS-C285728 - Provider product page
- Provider
- LSBio
- Product name
- ANXA1 / Annexin A1 Antibody (aa 5-346) LS-C285728
- Antibody type
- Polyclonal
- Description
- Caprylic acid and ammonium sulfate precipitation
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at -20°C or -80°C upon receipt.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot Positive WB detected in: PC-3 whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate All lanes: ANXA1 antibody at 2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39 kDa Observed band size: 39 kDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating ANXA1 in K562 whole cell lysate Lane 1: Rabbit control IgG instead of ANXA1 Antibody in K562 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: ANXA1 Antibody (8µg) + K562 whole cell lysate (500µg) Lane 3: K562 whole cell lysate (10µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating ANXA1 in K562 whole cell lysate Lane 1: Rabbit control IgG instead of ANXA1 Antibody in K562 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: ANXA1 Antibody (8µg) + K562 whole cell lysate (500µg) Lane 3: K562 whole cell lysate (10µg)