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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunoprecipitation [2]
- Immunohistochemistry [2]
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- Product number
- GTX101070 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101070, RRID:AB_2036236
- Product name
- Annexin A1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Hyaluronic acid-dependent protection in H9C2 cardiomyocytes: a cell model of heart ischemia-reperfusion injury and treatment.
Proteomic and redox-proteomic study on the role of glutathione reductase in human lung cancer cells.
Law CH, Li JM, Chou HC, Chen YH, Chan HL
Toxicology 2013 Jan 7;303:54-71
Toxicology 2013 Jan 7;303:54-71
Proteomic and redox-proteomic study on the role of glutathione reductase in human lung cancer cells.
Fan CY, Chou HC, Lo YW, Wen YF, Tsai YC, Huang H, Chan HL
Electrophoresis 2013 Dec;34(24):3305-14
Electrophoresis 2013 Dec;34(24):3305-14
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: NIH-3T3 B: JC 10% SDS PAGE GTX101070 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: A431 (GTX27909) B: H1299 C: HeLa 10% SDS PAGE GTX101070 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Annexin A1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX101070) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Annexin A1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX101070) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE GTX101070 diluted at 1:10000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Annexin I(GTX101070) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of Annexin A1 protein from HeLa whole cell extracts using 5 ?g of Annexin A1 antibody (GTX101070).Western blot analysis was performed using Annexin A1 antibody (GTX101070).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
- Validation comment
- IP
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of Annexin A1 protein from HeLa whole cell extracts using 5 £gg of Annexin A1 antibody (GTX101070).Western blot analysis was performed using Annexin A1 antibody (GTX101070).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded N87 Xenograft , using Annexin I(GTX101070) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Annexin A1 antibody detects Annexin A1 protein at cytosol and nucleus on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Annexin A1 antibody (GTX101070) dilution: 1:500.
