MAB3770

antibody from Novus Biologicals

Targeting: ANXA1 ANX1, LPC1

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB3770

Provider

Novus Biologicals

Product name

Mouse Monoclonal Annexin A1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Annexin A1 in direct ELISAs. Detects human and mouse Annexin A1 in Western blots. In direct ELISAs, no cross-reactivity with recombinant human Annexin A2, A3, A4, A6, A7, A8, A9, A10, A11, or A13 is observed.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Annexin A1 in Human Breast. Annexin A1 was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti-Human Annexin A1 Monoclonal Antibody (Catalog # MAB3770) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to myoepithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.