LS-C285447

antibody from LSBio

Targeting: ANXA1 ANX1, LPC1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C285447

Provider

LSBio

Product name

ANXA1 / Annexin A1 Antibody (aa2 - 346) LS-C285447

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Storage

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot All lanes: Anxa1 antibody at 2µg/ml Lane 1: PC-3 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 39 kDa Observed band size: 39 kDa

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western Blot Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, PC-3 whole cell lysate All lanes: Anxa1 antibody at 2.9µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39 kDa Observed band size: 39 kDa

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating Anxa1 in NIH/3T3 whole cell lysate Lane 1: Rabbit control IgG instead of Anxa1 Antibody in NIH/3T3 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: Anxa1 Antibody (8µg) + NIH/3T3 whole cell lysate (500µg) Lane 3: NIH/3T3 whole cell lysate (20µg)