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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- ABIN2616482 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Annexin A1 (ANXA1) (AA 1-346) antibody
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Mouse
- Epitope
- AA 1-346
- Isotype
- IgG
- Vial size
- 50 μg
- Storage
- May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
- Handling
- Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- RNA pol II CTD Ser2ph / Ser5ph antibody (mAb) (Clone 1A12G10) tested by Western blot. HeLa nuclear extract (30 μg per lane) probed with RNA pol II CTD Ser2ph / Ser5ph antibody (mAb) at a 1 μg/ml dilution.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- RNA pol II CTD Ser2ph / Ser5ph antibody (mAb) (Clone 1A12G10) tested by immunofluorescence. Left: HeLa cell stained with RNA pol II CTD Ser2ph / Ser5ph antibody (mAb). Middle:Hoechst. Right: Merge.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- RNA pol II CTD Ser2ph / Ser5ph antibody (mAb) (Clone 1A12G10) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 μg of chromatin from HL-60 cells and 4 μg of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 11 million sequence tags were mapped to identify RNA pol II CTD Ser2ph / Ser5ph binding sites. The image shows binding across a region of chromosome 15. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.
