H00000301-M10

antibody from Abnova Corporation

Targeting: ANXA1 ANX1, LPC1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000301-M10

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000301-M10, RRID:AB_1672255

Product name

ANXA1 monoclonal antibody (M10), clone 2D11

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant ANXA1.

Antigen sequence

MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSA
VSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILT
KRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLE
EVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEI
LASRTNKEIRDINRVYREELKRDLAKDITSDTSGD
FRNALLSLAKGDRSEDFGVNEDLADSDARALYEAG
ERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSK
HDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAE
KLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFY
QKMYGISLCQAILDETKGDYEKILVALCGGN


Isotype

IgG

Antibody clone number

2D11

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of ANXA1 expression in transfected 293T cell line by ANXA1 monoclonal antibody (M10), clone 2D11.Lane 1: ANXA1 transfected lysate(38.7 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

ANXA1 monoclonal antibody (M10), clone 2D11. Western Blot analysis of ANXA1 expression in human placenta.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

ANXA1 monoclonal antibody (M10), clone 2D11. Western Blot analysis of ANXA1 expression in HepG2(Cat # L019V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ANXA1 is 3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol