LS-B14983

antibody from LSBio

Targeting: ANXA1 ANX1, LPC1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B14983

Provider

LSBio

Product name

IHC-plus™ ANXA1 / Annexin A1 Antibody (aa 5-346) LS-B14983

Antibody type

Polyclonal

Description

Caprylic acid and ammonium sulfate precipitation

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at -20°C or -80°C upon receipt.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western Blot Positive WB detected in: PC-3 whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate All lanes: ANXA1 antibody at 2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39 kDa Observed band size: 39 kDa

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating ANXA1 in K562 whole cell lysate Lane 1: Rabbit control IgG instead of ANXA1 Antibody in K562 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: ANXA1 Antibody (8µg) + K562 whole cell lysate (500µg) Lane 3: K562 whole cell lysate (10µg)