LS-C755397

antibody from LSBio

Targeting: ANXA1 ANX1, LPC1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

LS-C755397

Provider

LSBio

Product name

ANXA1 / Annexin A1 Antibody (clone AT2G5) LS-C755397

Antibody type

Monoclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

AT2G5

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

The Recombinant Human Annexin A1 (10ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Annexin A1 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human Annexin A1 Lane 2.: HeLa cell lysate Lane 3.: A549 cell lysate Lane 4.: A431 cell lysate The HeLa cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Annexin A1 antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Anti- Annexin A1 antibody (1:10000) Lane 2.: Anti- Annexin A1 antibody (1:20000)