21990-1-AP

antibody from Proteintech Group

Targeting: ANXA1 ANX1, LPC1

Provider product page for 21990-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [32]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]
Immunohistochemistry [2]

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Product number: 21990-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#21990-1-AP, RRID:AB_11182596
Product name: Annexin A1 antibody
Antibody type: Polyclonal
Description: KD/KO validated Annexin A1 antibody (Cat. #21990-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

ANXA1 protein structure - 21990-1-AP shown in red.

Submitted references Comprehensive Analysis of Fatty Acid Metabolism in Diabetic Nephropathy from the Perspective of Immune Landscapes, Diagnosis and Precise Therapy.

Zhu E, Zhong M, Liang T, Liu Y, Wu K, Zhang Z, Zhao S, Guan H, Chen J, Zhang LZ, Zhang Y
Journal of inflammation research 2024;17:693-710

Tumorous IRE1α facilitates CD8(+)T cells-dependent anti-tumor immunity and improves immunotherapy efficacy in melanoma.

Yang Y, Wang S, Wang XX, Guo S, Wang H, Shi Q, Tian Y, Wang H, Zhao T, Zhang H, Zhang B, Gao T, Li C, Yi X, Guo W
Cell communication and signaling : CCS 2024 Jan 30;22(1):83

A pan-cancer single-cell panorama of human natural killer cells.

Tang F, Li J, Qi L, Liu D, Bo Y, Qin S, Miao Y, Yu K, Hou W, Li J, Peng J, Tian Z, Zhu L, Peng H, Wang D, Zhang Z
Cell 2023 Sep 14;186(19):4235-4251.e20

Effects on exocytosis by two HSV-1 mutants unable to block autophagy.

Waisner H, Lasnier S, Suma SM, Kalamvoki M
Journal of virology 2023 Oct 31;97(10):e0075723

The E3 ligase RBCK1 reduces the sensitivity of ccRCC to sunitinib through the ANKRD35-MITD1-ANXA1 axis.

Wang Y, Peng M, Zhong Y, Xiong W, Zhu L, Jin X
Oncogene 2023 Mar;42(13):952-966

Annexin A1 induces oxaliplatin resistance of gastric cancer through autophagy by targeting PI3K/AKT/mTOR.

Ren J, Hu Z, Niu G, Xia J, Wang X, Hong R, Gu J, Wang D, Ke C
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2023 Mar;37(3):e22790

Annexin A1 is a polarity cue that directs mitotic spindle orientation during mammalian epithelial morphogenesis.

Fankhaenel M, Hashemi FSG, Mourao L, Lucas E, Hosawi MM, Skipp P, Morin X, Scheele CLGJ, Elias S
Nature communications 2023 Jan 11;14(1):151

Proteomic analysis reveals the molecular mechanism of Astragaloside in the treatment of non-small cell lung cancer by inducing apoptosis.

Liu J, Sun Y, Chen W, Deng L, Chen M, Dong J
BMC complementary medicine and therapies 2023 Dec 15;23(1):461

Evolutionarily divergent mTOR remodels translatome for tissue regeneration.

Zhulyn O, Rosenblatt HD, Shokat L, Dai S, Kuzuoglu-Öztürk D, Zhang Z, Ruggero D, Shokat KM, Barna M
Nature 2023 Aug;620(7972):163-171

Development of a Novel Circulating Autoantibody Biomarker Panel for the Identification of Patients with 'Actionable' Pulmonary Nodules.

Auger C, Moudgalya H, Neely MR, Stephan JT, Tarhoni I, Gerard D, Basu S, Fhied CL, Abdelkader A, Vargas M, Hu S, Hulett T, Liptay MJ, Shah P, Seder CW, Borgia JA
Cancers 2023 Apr 12;15(8)

ANXA1: An Important Independent Prognostic Factor and Molecular Target in Glioma.

Zhang D, Wang W, Zhou H, Su L, Han X, Zhang X, Han W, Wang Y, Xue X
Frontiers in genetics 2022;13:851505

Circulating PD-L1 is associated with T cell infiltration and predicts prognosis in patients with CRLM following hepatic resection.

Chen X, Du Z, Huang M, Wang D, Fong WP, Liang J, Fan L, Wang Y, Yang H, Chen Z, Hu M, Xu R, Li Y
Cancer immunology, immunotherapy : CII 2022 Mar;71(3):661-674

Recruitment of DNA to tumor-derived microvesicles.

Clancy JW, Sheehan CS, Boomgarden AC, D'Souza-Schorey C
Cell reports 2022 Mar 1;38(9):110443

Reassessment of the Proteomic Composition and Function of Extracellular Vesicles in the Seminal Plasma.

Wang H, Zhu Y, Tang C, Zhou Z, Wang Z, Li Z, Zheng X, Chen S, Zhou Y, Liang A, Li Y, Lin Y, Sun F
Endocrinology 2022 Jan 1;163(1)

Crosstalk Between RPE Cells and Choroidal Endothelial Cells via the ANXA1/FPR2/SHP2/NLRP3 Inflammasome/Pyroptosis Axis Promotes Choroidal Neovascularization.

Zhu M, Wang Y, Zhu L, Du S, Wang Z, Zhang Y, Guo Y, Tu Y, Song E
Inflammation 2022 Feb;45(1):414-427

YTHDC1 is downregulated by the YY1/HDAC2 complex and controls the sensitivity of ccRCC to sunitinib by targeting the ANXA1-MAPK pathway.

Li W, Ye K, Li X, Liu X, Peng M, Chen F, Xiong W, Wang Y, Zhu L
Journal of experimental & clinical cancer research : CR 2022 Aug 17;41(1):250

Persistence of Lgr5+ colonic epithelial stem cells in mouse models of inflammatory bowel disease.

Girish N, Liu CY, Gadeock S, Gomez ML, Huang Y, Sharifkhodaei Z, Washington MK, Polk DB
American journal of physiology. Gastrointestinal and liver physiology 2021 Sep 1;321(3):G308-G324

ANXA1 affects murine hair follicle growth through EGF signaling pathway.

Zhang H, Fu X, Ao Y, Nan M, Qiu Z, Jia X, Xiao Y, Liu D, Guo X
Gene 2021 Mar 1;771:145343

Exosomal annexin A6 induces gemcitabine resistance by inhibiting ubiquitination and degradation of EGFR in triple-negative breast cancer.

Li T, Tao Z, Zhu Y, Liu X, Wang L, Du Y, Cao J, Wang B, Zhang J, Hu X
Cell death & disease 2021 Jul 8;12(7):684

Annexin A1 Attenuates Neutrophil Migration and IL-6 Expression through Fpr2 in a Mouse Model of Streptococcus suis-Induced Meningitis.

Ni C, Gao S, Zheng Y, Liu P, Zhai Y, Huang W, Jiang H, Lv Q, Kong D, Jiang Y
Infection and immunity 2021 Feb 16;89(3)

EphB2 activates CREB-dependent expression of Annexin A1 to regulate dendritic spine morphogenesis.

Yuan L, Yi W, Sun C, Ma S, Wang J, Liu S, Chen Y, Chen Y, Chen Y
Biochemical and biophysical research communications 2021 Dec 20;584:107-115

Global mapping of Salmonella enterica-host protein-protein interactions during infection.

Walch P, Selkrig J, Knodler LA, Rettel M, Stein F, Fernandez K, Viéitez C, Potel CM, Scholzen K, Geyer M, Rottner K, Steele-Mortimer O, Savitski MM, Holden DW, Typas A
Cell host & microbe 2021 Aug 11;29(8):1316-1332.e12

ER-luminal [Ca(2+)] regulation of InsP(3) receptor gating mediated by an ER-luminal peripheral Ca(2+)-binding protein.

Vais H, Wang M, Mallilankaraman K, Payne R, McKennan C, Lock JT, Spruce LA, Fiest C, Chan MY, Parker I, Seeholzer SH, Foskett JK, Mak DD
eLife 2020 May 18;9

WISP1 promotes bovine MDSC differentiation via recruitment of ANXA1 for the regulation of the TGF-β signalling pathway.

Zhang C, Zhang Y, Zhang W, Tong H, Li S, Yan Y
Molecular and cellular biochemistry 2020 Jul;470(1-2):215-227

Proteomic analysis of seminal extracellular vesicle proteins involved in asthenozoospermia by iTRAQ.

Lin Y, Liang A, He Y, Li Z, Li Z, Wang G, Sun F
Molecular reproduction and development 2019 Sep;86(9):1094-1105

TCEA3 promotes differentiation of C2C12 cells via an Annexin A1-mediated transforming growth factor-β signaling pathway.

Ge Y, Li S, Hu XY, Tong HL, Li SF, Yan YQ
Journal of cellular physiology 2019 Jul;234(7):10554-10565

Membrane Stabilization by Modified Steroid Offers a Potential Therapy for Muscular Dystrophy Due to Dysferlin Deficit.

Sreetama SC, Chandra G, Van der Meulen JH, Ahmad MM, Suzuki P, Bhuvanendran S, Nagaraju K, Hoffman EP, Jaiswal JK
Molecular therapy : the journal of the American Society of Gene Therapy 2018 Sep 5;26(9):2231-2242

Enhanced antitumor effect of combination of annexin A1 knockdown and bortezomib treatment in multiple myeloma in vitro and in vivo.

Jia C, Kong D, Guo Y, Li L, Quan L
Biochemical and biophysical research communications 2018 Nov 2;505(3):720-725

Participation of delta annexin A3 in the ribosomal protein S19 C-terminus-dependent inhibitory mechanism of the neutrophil C5a receptor through delta lactoferrin.

Yamanegi K, Yamada N, Nakasho K, Nishiura H
Pathology international 2018 Feb;68(2):109-116

Dynamic expression patterns of differential proteins during early invasion of hepatocellular carcinoma.

Chen RX, Song HY, Dong YY, Hu C, Zheng QD, Xue TC, Liu XH, Zhang Y, Chen J, Ren ZG, Liu YK, Cui JF
PloS one 2014;9(3):e88543

Identification of protein network alterations upon retinal ischemia-reperfusion injury by quantitative proteomics using a Rattus norvegicus model.

Tian H, Wang L, Cai R, Zheng L, Guo L
PloS one 2014;9(12):e116453

Proteomic investigation of anti-tumor activities exerted by sinularin against A2058 melanoma cells.

Su TR, Lin JJ, Chiu CC, Chen JY, Su JH, Cheng ZJ, Hwang WI, Huang HH, Wu YJ
Electrophoresis 2012 Apr;33(7):1139-52

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: PC-3 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP(ANXA1 antibody) at dilution of 1:2000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The Annexin A1 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human Annexin A1. This antibody recognizes human,mouse,rat antigen. The Annexin A1 antibody has been validated for the following applications: ELISA, WB, IHC, IF, IP analysis.
Appendix: 21990-1-AP.pdf

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-ANXA1 (IP:21990-1-AP, 3ug; Detection:21990-1-AP 1:2000) with HeLa cells lysate 2400ug.
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human lung using 21990-1-AP(ANXA1 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human lung using 21990-1-AP(ANXA1 antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue