Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-22266 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Annexin A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: H1299, NIH-3T3, HeLa. Predicted reactivity: Mouse (86%), Rat (89%), Dog (92%), Cat (93%), Pig (87%), Rabbit (92%), Chimpanzee (99%), Bovine (89%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Cardiac Alarmins as Residual Risk Markers of Atherosclerosis under Hypolipidemic Therapy.
Suica VI, Uyy E, Ivan L, Boteanu RM, Cerveanu-Hogas A, Hansen R, Antohe F
International journal of molecular sciences 2022 Sep 22;23(19)
International journal of molecular sciences 2022 Sep 22;23(19)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Annexin A1 Polyclonal Antibody (Product # PA5-22266). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. 10% SDS PAGE. Annexin A1 Polyclonal Antibody (Product # PA5-22266) diluted at 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Annexin A1 Polyclonal Antibody (Product # PA5-22266). Sample (30 µg of whole cell lysate). Lane A: H1299. 10% SDS PAGE. Annexin A1 Polyclonal Antibody (Product # PA5-22266) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Annexin A1 in paraformaldehyde-fixed HeLa cells using an Annexin A1 polyclonal antibody (Product # PA5-22266) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Annexin A1 (Product # PA5-22266) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.