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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- NBP2-02357 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Annexin A1 Antibody
- Antibody type
- Monoclonal
- Description
- Affinity purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Annexin A1 Antibody (3A8) [NBP2-02357] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Annexin A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Annexin A1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Annexin A1 Antibody (OTI3A8) [NBP2-02357] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and ANXA1-Knockout hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-ANXA1 monoclonal antibody. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody as a loading control (1:500).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Annexin A1 Antibody (3A8) [NBP2-02357] - Staining of paraffin-embedded Kidney tissue using anti-Annexin A1 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Annexin A1 Antibody (3A8) [NBP2-02357] - Staining of paraffin-embedded breast tissue using anti-Annexin A1 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Annexin A1 Antibody (3A8) [NBP2-02357] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-Annexin A1 mouse monoclonal antibody.
