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- Antibody Data
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- Validations
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-27315 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Annexin A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, NIH-3T3, JC, ANXA1-transfected 293T. Predicted reactivity: Mouse (86%), Rat (88%), Dog (91%), Pig (88%), Rabbit (91%), Chimpanzee (100%), Bovine (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.95 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Annexin A1 protects against cerebral ischemia-reperfusion injury by modulating microglia/macrophage polarization via FPR2/ALX-dependent AMPK-mTOR pathway.
Lineage Reversion Drives WNT Independence in Intestinal Cancer.
Xu X, Gao W, Li L, Hao J, Yang B, Wang T, Li L, Bai X, Li F, Ren H, Zhang M, Zhang L, Wang J, Wang D, Zhang J, Jiao L
Journal of neuroinflammation 2021 May 22;18(1):119
Journal of neuroinflammation 2021 May 22;18(1):119
Lineage Reversion Drives WNT Independence in Intestinal Cancer.
Han T, Goswami S, Hu Y, Tang F, Zafra MP, Murphy C, Cao Z, Poirier JT, Khurana E, Elemento O, Hechtman JF, Ganesh K, Yaeger R, Dow LE
Cancer discovery 2020 Oct;10(10):1590-1609
Cancer discovery 2020 Oct;10(10):1590-1609
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Annexin A1 in paraformaldehyde-fixed HeLa cells using an Annexin A1 polyclonal antibody (Product # PA5-27315) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Annexin A1 Polyclonal Antibody detects Annexin A1 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Annexin A1 stained by Annexin A1 Polyclonal Antibody (Product # PA5-27315) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Annexin A1 was performed in HeLa whole cell extracts using 5 µg of Annexin A1 Polyclonal Antibody (Product # PA5-27315). Samples were transferred to a membrane and probed with Annexin A1 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Annexin A1 Polyclonal Antibody detects Annexin A1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Annexin A1 stained by Annexin A1 Polyclonal Antibody (Product # PA5-27315) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded N87 Xenograft , using Annexin I (Product # PA5-27315) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Annexin A1 was performed in HeLa whole cell extracts using 5 µg of Annexin A1 Polyclonal Antibody (Product # PA5-27315). Samples were transferred to a membrane and probed with Annexin A1 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
