PA5-27315

antibody from Invitrogen Antibodies

Targeting: ANXA1 ANX1, LPC1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27315

Provider

Invitrogen Antibodies

Product name

Annexin A1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HeLa, NIH-3T3, JC, ANXA1-transfected 293T. Predicted reactivity: Mouse (86%), Rat (88%), Dog (91%), Pig (88%), Rabbit (91%), Chimpanzee (100%), Bovine (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.95 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Annexin A1 protects against cerebral ischemia-reperfusion injury by modulating microglia/macrophage polarization via FPR2/ALX-dependent AMPK-mTOR pathway.

Xu X, Gao W, Li L, Hao J, Yang B, Wang T, Li L, Bai X, Li F, Ren H, Zhang M, Zhang L, Wang J, Wang D, Zhang J, Jiao L
Journal of neuroinflammation 2021 May 22;18(1):119

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Lineage Reversion Drives WNT Independence in Intestinal Cancer.

Han T, Goswami S, Hu Y, Tang F, Zafra MP, Murphy C, Cao Z, Poirier JT, Khurana E, Elemento O, Hechtman JF, Ganesh K, Yaeger R, Dow LE
Cancer discovery 2020 Oct;10(10):1590-1609

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Annexin A1 using 30 µg of A) A431 (B) H1299 and C) HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an Annexin A1 polyclonal antibody (Product # PA5-27315) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Annexin A1 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Annexin A1 Polyclonal Antibody (Product # PA5-27315) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Annexin A1 Polyclonal Antibody (Product # PA5-27315). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. Lane B: JC. 10% SDS PAGE. Annexin A1 Polyclonal Antibody (Product # PA5-27315) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Annexin A1 Polyclonal Antibody (Product # PA5-27315). Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 Polyclonal Antibody (Product # PA5-27315) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Annexin A1 in paraformaldehyde-fixed HeLa cells using an Annexin A1 polyclonal antibody (Product # PA5-27315) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Annexin A1 Polyclonal Antibody detects Annexin A1 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Annexin A1 stained by Annexin A1 Polyclonal Antibody (Product # PA5-27315) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Annexin A1 was performed in HeLa whole cell extracts using 5 µg of Annexin A1 Polyclonal Antibody (Product # PA5-27315). Samples were transferred to a membrane and probed with Annexin A1 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.