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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-27315 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Annexin A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, NIH-3T3, JC, ANXA1-transfected 293T.
- Concentration
- 0.95 mg/mL
Submitted references Annexin A1 protects against cerebral ischemia-reperfusion injury by modulating microglia/macrophage polarization via FPR2/ALX-dependent AMPK-mTOR pathway.
Lineage Reversion Drives WNT Independence in Intestinal Cancer.
Xu X, Gao W, Li L, Hao J, Yang B, Wang T, Li L, Bai X, Li F, Ren H, Zhang M, Zhang L, Wang J, Wang D, Zhang J, Jiao L
Journal of neuroinflammation 2021 May 22;18(1):119
Journal of neuroinflammation 2021 May 22;18(1):119
Lineage Reversion Drives WNT Independence in Intestinal Cancer.
Han T, Goswami S, Hu Y, Tang F, Zafra MP, Murphy C, Cao Z, Poirier JT, Khurana E, Elemento O, Hechtman JF, Ganesh K, Yaeger R, Dow LE
Cancer discovery 2020 Oct;10(10):1590-1609
Cancer discovery 2020 Oct;10(10):1590-1609
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Annexin A1 using 30 µg of A) A431 (B) H1299 and C) HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an Annexin A1 polyclonal antibody (Product # PA5-27315) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Annexin A1 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Annexin A1 Polyclonal Antibody (Product # PA5-27315) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Annexin A1 Polyclonal Antibody (Product # PA5-27315). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. Lane B: JC. 10% SDS PAGE. Annexin A1 Polyclonal Antibody (Product # PA5-27315) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Annexin A1 Polyclonal Antibody (Product # PA5-27315). Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 Polyclonal Antibody (Product # PA5-27315) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Annexin A1 in paraformaldehyde-fixed HeLa cells using an Annexin A1 polyclonal antibody (Product # PA5-27315) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Annexin A1 Polyclonal Antibody detects Annexin A1 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Annexin A1 stained by Annexin A1 Polyclonal Antibody (Product # PA5-27315) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Annexin A1 Polyclonal Antibody detects Annexin A1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Annexin A1 stained by Annexin A1 Polyclonal Antibody (Product # PA5-27315) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded N87 Xenograft , using Annexin I (Product # PA5-27315) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Annexin A1 was performed in HeLa whole cell extracts using 5 µg of Annexin A1 Polyclonal Antibody (Product # PA5-27315). Samples were transferred to a membrane and probed with Annexin A1 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
