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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MAB3770 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Annexin A1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Annexin A1 in direct ELISAs. Detects human and mouse Annexin A1 in Western blots. In direct ELISAs, no cross-reactivity with recombinant human Annexin A2, A3, A4, A6, A7, A8, A9, A10, A11, or A13 is observed.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P04083- Isotype
- IgG
- Antibody clone number
- 686106
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Annexin A1 in NIH-3T3 Mouse Cell Line. Annexin A1 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line (left panel, positive stain) and NIH-3T3 Knockouts (right panel, negative stain) using Mouse Anti-Human/Mouse Annexin A1 Monoclonal Antibody (Catalog # MAB3770) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei, cytoplasm and plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Annexin A1 in Human Breast. Annexin A1 was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti-Human/Mouse Annexin A1 Monoclonal Antibody (Catalog # MAB3770) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to myoepithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
