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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- MAB37701 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Annexin A1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Annexin A1 in direct ELISAs and human, mouse, and rat Annexin A1 in Western blots. In direct ELISAs, approximately 25%-50% cross-reactivity with recombinant human (rh) Annexin A2 is observed and no cross-reactivity with rhAnnexin A3, A6, or A11 is observed. In Western blots, no cross-reactivity with rhAnnexin A6 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P04083- Isotype
- IgG
- Antibody clone number
- 686122
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat Annexin A1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse Annexin A1 Monoclonal Antibody (Catalog # MAB37701) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Annexin A1 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Annexin A1 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Annexin A1 at approximately 45 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat Annexin A1 Monoclonal Antibody (Catalog # MAB37701). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
